Evidence map›Paper›PMID 29696078›Full record

ArticleJournal of extracellular vesicles2018

Newt cells secrete extracellular vesicles with therapeutic bioactivity in mammalian cardiomyocytes.

Ryan C Middleton, Russell G Rogers, Geoffrey De Couto, Eleni Tseliou, Kristin Luther, Ronald Holewinski, Daniel Soetkamp, Jennifer E Van Eyk, Travis J Antes, Eduardo Marbán

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 40 citations in OpenAlex.

  1. Review
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  5. Proteomics of the heart.Physiological reviews · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Ryan C MiddletonSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Russell G RogersSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Geoffrey De CoutoSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Eleni TseliouSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Kristin LutherSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Ronald HolewinskiSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Daniel SoetkampSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Jennifer E Van EykSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Travis J AntesSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Eduardo MarbánSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Cedars-Sinai Smidt Heart Institute · USCedars-Sinai Medical Center · US

Funding

Exosome-mediated cardioprotection and regenerationR01HL124074 · NHLBI · CEDARS-SINAI MEDICAL CENTER · PI MARBAN, EDUARDO · 2014 to 2021
$4.0M
NHLBI NIH HHS R01 HL124074
6 · The paper itself

Abstract

Newts can regenerate amputated limbs and cardiac tissue, unlike mammals which lack broad regenerative capacity. Several signaling pathways involved in cell proliferation, differentiation and survival during newt tissue regeneration have been elucidated, however the factors that coordinate signaling between cells, as well as the conservation of these factors in other animals, are not well defined. Here we report that media conditioned by newt limb explant cells (A1 cells) protect mammalian cardiomyocytes from oxidative stress-induced apoptosis. The cytoprotective effect of A1-conditioned media was negated by exposing A1 cells to GW4869, which suppresses the generation of extracellular vesicles (EVs). A1-EVs are similar in diameter (~100-150 nm), structure, and share several membrane surface and cargo proteins with mammalian exosomes. However, isolated A1-EVs contain significantly higher levels of both RNA and protein per particle than mammalian EVs. Additionally, numerous cargo RNAs and proteins are unique to A1-EVs. Of particular note, A1-EVs contain numerous mRNAs encoding nuclear receptors, membrane ligands, as well as transcription factors. Mammalian cardiomyocytes treated with A1-EVs showed increased expression of genes in the PI3K/AKT pathway, a pivotal player in survival signaling. We conclude that newt cells secrete EVs with diverse, distinctive RNA and protein contents. Despite ~300 million years of evolutionary divergence between newts and mammals, newt EVs confer cytoprotective effects on mammalian cardiomyocytes.

Indexed as

apoptosiscardiomyocytescytoprotectionExosomesextracellular vesiclesnewtPI3K/AKT pathwaytissue regenerationUrodele amphibians

Identifiers

PMID29696078
PMCPMC5912190
OpenAlexW2800903814

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.