ArticleScientific reports2018
Novel Type III Polyketide Synthases Biosynthesize Methylated Polyketides in Mycobacterium marinum.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Genome-wide analysis of biosynthetic gene clusters reveals hidden metabolic diversity in bacterial fish pathogens.World journal of microbiology & biotechnology · 2026Article
- Mycobacterium smegmatis Expands Across Surfaces by Hydraulic Sliding.Environmental microbiology reports · 2025Article
- Functional flexibility of a type III polyketide synthase iniScience · 2025Article
- Designing a highly efficient type III polyketide whole-cell catalyst with minimized byproduct formation.Biotechnology for biofuels and bioproducts · 2024Article
- Deciphering the genomic character of the multidrug-resistantAIMS microbiology · 2024Article
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- The pan-genome of Mycobacterium avium subsp. paratuberculosis (Map) confirms ancestral lineage and reveals gene rearrangements within Map Type S.BMC genomics · 2023Article
- Extreme summers impact cropland and grassland soil microbiomes.The ISME journal · 2023Article
- Improved polyketide production in C. glutamicum by preventing propionate-induced growth inhibition.Nature metabolism · 2023Article
- A Type III Polyketide Synthase Specific for Sporulating Negativicutes is Responsible for Alkylpyrone Biosynthesis.Chembiochem : a European journal of chemical biology · 2022Article
- Article
- Reconstruction of Secondary Metabolic Pathway to Synthesize Novel Metabolite inFrontiers in bioengineering and biotechnology · 2021Article
- Review
- Chemistry, bioactivity and biosynthesis of cyanobacterial alkylresorcinols.Natural product reports · 2019Review
- Cytochrome P450 Monooxygenase CYP139 Family Involved in the Synthesis of Secondary Metabolites in 824 Mycobacterial Species.International journal of molecular sciences · 2019Article
- Genome mining reveals uncommon alkylpyrones as type III PKS products from myxobacteria.Journal of industrial microbiology & biotechnology · 2019Article
- Antimicrobial potentiality of actinobacteria isolated from two microbiologically unexplored forest ecosystems of Northeast India.BMC microbiology · 2018Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterial pathogenesis is hallmarked by lipidic polyketides that decorate the cell envelope and mediate infection. However, factors mediating persistence remain largely unknown. Dynamic cell wall remodeling could facilitate the different pathogenic phases. Recent studies have implicated type III polyketide synthases (PKSs) in cell wall alterations in several bacteria. Comparative genome analysis revealed several type III pks gene clusters in mycobacteria. In this study, we report the functional characterization of two novel type III PKSs, MMAR_2470 and MMAR_2474, in Mycobacterium marinum. These type III pkss belong to a unique pks genomic cluster conserved exclusively in pathogenic mycobacteria. Cell-free reconstitution assays and high-resolution mass spectrometric analyses revealed methylated polyketide products in independent reactions of both proteins. MMAR_2474 protein exceptionally biosynthesized methylated alkyl-resorcinol and methylated acyl-phloroglucinol products from the same catalytic core. Structure-based homology modeling, product docking, and mutational studies identified residues that could facilitate the distinctive catalysis of these proteins. Functional investigations in heterologous mycobacterial strain implicated MMAR_2474 protein to be vital for mycobacterial survival in stationary biofilms. Our investigations provide new insights into type III PKSs conserved in pathogenic mycobacterial species.
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