ArticleScientific reports2018
Label-free non-invasive quantitative measurement of lipid contents in individual microalgal cells using refractive index tomography.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Beyond conventional imaging: curvelet-AI convergence for non-invasive microalgal lipid quantification and biofuel optimization.Bioprocess and biosystems engineering · 2026Review
- Optical diffraction tomographic microscopy: a cutting-edge label-free three-dimensional bioimaging.Biophysics reports · 2026Article
- Quantitative holographic analysis in stallion spermatozoa following cryopreservation.Scientific reports · 2025Article
- Polarization-Sensitive Holotomography for Multidimensional Label-Free Imaging and Characterization of Lipid Droplets in Cancer Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Moderate levels of dissolved iron stimulate cellular growth and increase lipid storage in Symbiodinium sp.Journal of phycology · 2025Article
- Holographic tomography of the diatom Skeletonema pseudocostatum used as a bioindicator of heavy metal-polluted waters.PloS one · 2025Article
- Integrating holotomography and deep learning for rapid detection of NPM1 mutations in AML.Scientific reports · 2024Article
- Low-dose ionizing radiation generates a hormetic response to modify lipid metabolism in Chlorella sorokiniana.Communications biology · 2024Article
- Quantitative phase imaging based on holography: trends and new perspectives.Light, science & applications · 2024Review
- Holotomography and atomic force microscopy: a powerful combination to enhance cancer, microbiology and nanotoxicology research.Discover nano · 2024Review
- Noninvasive morpho-molecular imaging reveals early therapy-induced senescence in human cancer cells.Science advances · 2023Article
- Real-Time Monitoring of Multitarget Antimicrobial Mechanisms of Peptoids Using Label-Free Imaging with Optical Diffraction Tomography.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Investigation of refractive index dynamics duringBiomedical optics express · 2023Article
- Advanced Microscopy Techniques for Molecular Biophysics.International journal of molecular sciences · 2023Review
- OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis.Nature cell biology · 2023Article
- Review
- Machine learning techniques to characterize functional traits of plankton from image data.Limnology and oceanography · 2022Review
- Interactions of Nanoparticles with Macrophages and Feasibility of Drug Delivery for Asthma.International journal of molecular sciences · 2022Article
- Spatial light interference microscopy: principle and applications to biomedicine.Advances in optics and photonics · 2021Article
- Bacteria Single-Cell and Photosensitizer Interaction Revealed by Quantitative Phase Imaging.International journal of molecular sciences · 2021Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microalgae are promising candidates for biofuel production due to their high lipid content. To facilitate utilization of the microalgae for biofuel, rapid quantification of the lipid contents in microalgae is necessary. However, conventional methods based on the chemical extraction of lipids require a time-consuming destructive extraction process. Here, we demonstrate label-free, non-invasive, rapid quantification of the lipid contents in individual micro-algal cells measuring the three-dimensional refractive index tomograms. We measure three-dimensional refractive index distributions within Nannochloropsis oculata cells and find that lipid droplets are identifiable in tomograms by their high refractive index. In addition, we alter N. oculata under nitrogen deficiency by measuring the volume, lipid weight, and dry cell weight of individual cells. Characterization of individual cells allows correlative analysis between the lipid content and size of individual cells.
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