ArticlemSphere2018
Rapid Gene Concatenation for Genetic Rescue of Multigene Mutants in
Article in mSphere, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Advancements in CRISPR-Cas Systems for Genome Editing towards Eradication of Human Microbial Pathogens.Molecular biotechnology · 2026Review
- Strain variation in themSphere · 2024Article
- Biofilm-associated metabolism via ERG251 in Candida albicans.PLoS pathogens · 2024Article
- Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection.PLoS pathogens · 2023Article
- Article
- Article
- Targeted Genetic Changes in Candida albicans Using Transient CRISPR-Cas9 Expression.Current protocols · 2021Article
- How to Use a Mutant Library to Identify Genes Required for Biofilm Formation in the PathogenicUJEMI+ · 2020Article
- Roles of Candida albicans Mig1 and Mig2 in glucose repression, pathogenicity traits, and SNF1 essentiality.PLoS genetics · 2020Article
- Circuit diversification in a biofilm regulatory network.PLoS pathogens · 2019Article
- Recent advances in understandingF1000Research · 2019Review
- CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The biological function of a gene is often probed through its interactions with other genes. This general approach has been especially useful to build knowledge about poorly understood genes upon the bedrock of well-characterized genes. Genetic interaction analysis requires the construction of strains with mutations in two or more genes. Single-gene mutants of microbial pathogens are generally validated through introduction of a wild-type copy of the affected gene to create a complemented or reconstituted strain, followed by testing for restoration of a wild-type phenotype. This practice, formalized as one of Falkow's "molecular Koch's postulates" ensures that the phenotype of the mutant depends upon the known mutation. However, multigene mutants are seldom validated because of the labor required to assemble multiple genomic segments into a vector that can be introduced into the mutant strain. We present here an approach, concatemer assembly for rescue of mutant abilities (CARMA), that circumvents this impediment through an
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.