Evidence map›Paper›PMID 29695620›Full record

ArticlemSphere2018

Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera.

A Balloch, L Roalfe, N Ekstrom, C D Nguyen, L Spry, R A Marimla, P V Licciardi, D Goldblatt, E K Mulholland

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in mSphere, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

A BallochPneumococcal Immunology Laboratory, Murdoch Children's Research Institute, Melbourne, Australia anne.balloch@mcri.edu.au.
L RoalfeUCL Great Ormond Street Institute of Child Health, London, United Kingdom.
N EkstromThe National Institute for Health and Welfare, Helsinki, Finland.
C D NguyenPneumococcal Immunology Laboratory, Murdoch Children's Research Institute, Melbourne, Australia.
L SpryPneumococcal Immunology Laboratory, Murdoch Children's Research Institute, Melbourne, Australia.
R A MarimlaPneumococcal Immunology Laboratory, Murdoch Children's Research Institute, Melbourne, Australia.
P V LicciardiPneumococcal Immunology Laboratory, Murdoch Children's Research Institute, Melbourne, Australia.
D GoldblattUCL Great Ormond Street Institute of Child Health, London, United Kingdom.
E K MulhollandPneumococcal Immunology Laboratory, Murdoch Children's Research Institute, Melbourne, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Opsonophagocytic assays are used to measure functional antibodies important in protection against pneumococcal capsular antigens. There have been efforts to standardize these methods, as the assays are commonly used to measure vaccine immunogenicity. We report here the results from three international laboratories using their own methods, based on the recommended WHO standard method. We tested 30 pediatric sera, before and after administration of a 13-valent conjugate pneumococcal vaccine, against all 13 serotypes. The three laboratories demonstrated good agreement using their own standardized multiplex opsonophagocytosis assay protocols, particularly postimmunization for those serotypes in the vaccine. While serotype-specific IgG methods have already been internationally standardized and are currently used as a measure of vaccine immunogenicity, this report demonstrates that despite minor differences in methods and a minor variation in response to nonvaccine serotypes, the results from opsonophagocytic assays across the three laboratories may be compared with confidence.

Indexed as

Antibodies, BacterialAntigens, BacterialChildChild, PreschoolHeptavalent Pneumococcal Conjugate VaccineHumansImmunogenicity, VaccineImmunoglobulin GImmunologic TestsOpsonin ProteinsPhagocytosisPneumococcal InfectionsPneumococcal VaccinesReproducibility of ResultsSerogroupStreptococcus pneumoniae13-valent pneumococcal vaccineAntibodies, BacterialAntigens, BacterialHeptavalent Pneumococcal Conjugate VaccineImmunoglobulin GOpsonin ProteinsPneumococcal VaccinesimmunizationMOPApneumococcal IgG

Identifiers

PMID29695620
PMCPMC5917425

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.