ArticleBiophysical journal2018
FRET Detects the Size of Nanodomains for Coexisting Liquid-Disordered and Liquid-Ordered Phases.
Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Advances in FRET-based Applications Within Biomimicking Molecular Self-assemblies.Journal of fluorescence · 2026Review
- Nanodomain formation in lipid bilayers II: The influence of mixed-chain saturated lipids.Biochimica et biophysica acta. Biomembranes · 2026Article
- Nanodomain formation in lipid bilayers I: Quantifying the nanoscopic miscibility transition with FRET.Biochimica et biophysica acta. Biomembranes · 2026Article
- Nanodomain formation in lipid bilayers II: The influence of mixed-chain saturated lipids.bioRxiv : the preprint server for biology · 2026Article
- Nanodomain formation in lipid bilayers I: Quantifying the nanoscopic miscibility transition with FRET.bioRxiv : the preprint server for biology · 2026Article
- Effect of a scramblase activator upon lipid and probe scrambling and membrane domain formation in HEK 293T cells.Faraday discussions · 2025Article
- The Importance of Bilayer Asymmetry in Biological Membranes: Insights from Model Membranes.Membranes · 2025Review
- Cryo-EM images of phase-separated lipid bilayer vesicles analyzed with a machine-learning approach.Biophysical journal · 2024Article
- Polar Glycerolipids and Membrane Lipid Rafts.International journal of molecular sciences · 2024Review
- Experimentally determined leaflet-leaflet phase diagram of an asymmetric lipid bilayer.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Eukaryotic Cell Membranes: Structure, Composition, Research Methods and Computational Modelling.International journal of molecular sciences · 2023Review
- Interleaflet organization of membrane nanodomains: What can(not) be resolved by FRET?Biophysical journal · 2023Article
- Nano-scale domains in the plasma membrane are like macroscopic domains in asymmetric bilayers.Biophysical journal · 2023Review
- Biophysical studies of lipid nanodomains using different physical characterization techniques.Biophysical journal · 2023Review
- Characterization of Phase Separated Planar Lipid Bilayer Membrane by Fluorescence Ratio Imaging and Scanning Probe Microscope.Membranes · 2022Article
- Molecular substructure of the liquid-ordered phase formed by sphingomyelin and cholesterol: sphingomyelin clusters forming nano-subdomains are a characteristic feature.Biophysical reviews · 2022Article
- Article
- Dynamic "Molecular Portraits" of Biomembranes Drawn by Their Lateral Nanoscale Inhomogeneities.International journal of molecular sciences · 2021Review
- Investigation of the domain line tension in asymmetric vesicles prepared via hemifusion.Biochimica et biophysica acta. Biomembranes · 2021Article
- Sphingomyelins and ent-Sphingomyelins Form Homophilic Nano-Subdomains within Liquid Ordered Domains.Biophysical journal · 2020Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Biomembranes with as few as three lipid components can form coexisting liquid-disordered (Ld) and liquid-ordered (Lo) phases. In the coexistence region of Ld and Lo phases, the lipid mixtures 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)/1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/chol or brain sphingomyelin (bSM)/DOPC/chol form micron-scale domains that are easily visualized with light microscopy. Although large domains are not observed in the mixtures DSPC/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/chol and bSM/POPC/chol, lateral heterogeneity is nevertheless detected using techniques with nanometer-scale spatial resolution. We propose a simple and accessible method to measure domain sizes below optical resolution (∼200 nm). We measured nanodomain size for the latter two mixtures by combining experimental Förster resonance energy transfer data with a Monte-Carlo-based analysis. We found a domain radius of 7.5-10 nm for DSPC/POPC/chol, similar to values obtained previously by neutron scattering, and ∼5 nm for bSM/POPC/chol, slightly smaller than measurable by neutron scattering. These analyses also detect the domain-size transition that is observed by fluorescence microscopy in the four-component lipid mixture bSM/DOPC/POPC/chol. Accurate measurements of fluorescent-probe partition coefficients are especially important for the analysis; therefore, we exploit three different methods to measure the partition coefficient of fluorescent molecules between Ld and Lo phases.
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