ArticleInternational journal of molecular sciences2018
Influence of Mechanical Unloading on Articular Chondrocyte Dedifferentiation.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Standardized Effect Measures Informing Next-Generation Strategies for Mechanical Stimulation in Cartilage Tissue Engineering.Advanced healthcare materials · 2026Pooled it
- Characterization of the random positioning machine as a microgravity simulator for biological applications.PloS one · 2026Article
- Antioxidant functionalized double-net/TA dynamic hydrogel promotes cartilage regeneration through stabilization of chondrocyte phenotype.Materials today. Bio · 2025Article
- Blocking calcium-MYC regulatory axis inhibits early dedifferentiation of chondrocytes and contributes to cartilage regeneration.Stem cell research & therapy · 2025Article
- Impact of Abnormal Mechanical Stress on Chondrocyte Death in Osteoarthritis.Medical science monitor : international medical journal of experimental and clinical research · 2025Review
- LIPUS Promotes Calcium Oscillation and Enhances Calcium Dependent Autophagy of Chondrocytes to Alleviate Osteoarthritis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Metabolic Profiles of Encapsulated Chondrocytes Exposed to Short-Term Simulated Microgravity.Annals of biomedical engineering · 2025Article
- Ion channels in osteoarthritis: emerging roles and potential targets.Nature reviews. Rheumatology · 2024Review
- Metabolic Profiles of Encapsulated Chondrocytes Exposed to Short-Term Simulated Microgravity.bioRxiv : the preprint server for biology · 2024Article
- LGR5 Modulates Differentiated Phenotypes of Chondrocytes Through PI3K/AKT Signaling Pathway.Tissue engineering and regenerative medicine · 2024Article
- Treatment of knee cartilage lesions in 2024: From hyaluronic acid to regenerative medicine.Journal of experimental orthopaedics · 2024Review
- Regulation of Oxygen Tension as a Strategy to Control Chondrocytic Phenotype for Cartilage Tissue Engineering and Regeneration.Bioengineering (Basel, Switzerland) · 2024Review
- Promoting Articular Cartilage Regeneration through Microenvironmental Regulation.Journal of immunology research · 2024Review
- Human Osteoarthritic Chondrocytes Express Nineteen Different TRP-Genes-TRPA1 and TRPM8 as Potential Drug Targets.International journal of molecular sciences · 2023Article
- Review
- Effect of electrohydrodynamic printing scaffold with different spacing on chondrocyte dedifferentiation.Annals of translational medicine · 2022Article
- 3D Printing for Bone-Cartilage Interface Regeneration.Frontiers in bioengineering and biotechnology · 2022Review
- The Role of Mechanically-Activated Ion Channels Piezo1, Piezo2, and TRPV4 in Chondrocyte Mechanotransduction and Mechano-Therapeutics for Osteoarthritis.Frontiers in cell and developmental biology · 2022Review
- Engineered Human Meniscus in Modeling Sex Differences of Knee OsteoarthritisFrontiers in bioengineering and biotechnology · 2022Article
- Mechanosensory and mechanotransductive processes mediated by ion channels in articular chondrocytes: Potential therapeutic targets for osteoarthritis.Channels (Austin, Tex.) · 2021Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the limited self-repair capacity of articular cartilage, the surgical restoration of defective cartilage remains a major clinical challenge. The cell-based approach, which is known as autologous chondrocyte transplantation (ACT), has limited success, presumably because the chondrocytes acquire a fibroblast-like phenotype in monolayer culture. This unwanted dedifferentiation process is typically addressed by using three-dimensional scaffolds, pellet culture, and/or the application of exogenous factors. Alternative mechanical unloading approaches are suggested to be beneficial in preserving the chondrocyte phenotype. In this study, we examined if the random positioning machine (RPM) could be used to expand chondrocytes in vitro such that they maintain their phenotype. Bovine chondrocytes were exposed to (a) eight days in static monolayer culture; (b) two days in static monolayer culture, followed by six days of RPM exposure; and, (c) eight days of RPM exposure. Furthermore, the experiment was also conducted with the application of 20 mM gadolinium, which is a nonspecific ion-channel blocker. The results revealed that the chondrocyte phenotype is preserved when chondrocytes go into suspension and aggregate to cell clusters. Exposure to RPM rotation alone does not preserve the chondrocyte phenotype. Interestingly, the gene expression (mRNA) of the mechanosensitive ion channel
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.