ArticlePlant methods2018
Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants.
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Bioorthogonal chemistry for deciphering lipid biology in living systems.RSC chemical biology · 2026Review
- From Molecule to Meaning: Click and Bioorthogonal Chemical Reporters for Plant Systems, Biological Imaging, and Artistic Expression.Chembiochem : a European journal of chemical biology · 2026Review
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Imaging Plant Lipids with Fluorescent Reporters.Plants (Basel, Switzerland) · 2024Review
- Exploring Lignification Complexity in Plant Cell Walls with Airyscan Super-resolution Microscopy and Bioorthogonal Chemistry.Chemical & biomedical imaging · 2023Article
- Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants.JACS Au · 2023Article
- Cell-derived membrane biomimetic nanocarriers for targeted therapy of pulmonary disease.International journal of pharmaceutics · 2022Review
- Imaging the living plant cell: From probes to quantification.The Plant cell · 2022Review
- Tracing Lipid Metabolism by Alkyne Lipids and Mass Spectrometry: The State of the Art.Frontiers in molecular biosciences · 2022Review
- Bioimaging tools move plant physiology studies forward.Frontiers in plant science · 2022Article
- Emerging Approaches to Functionalizing Cell Membrane-Coated Nanoparticles.Biochemistry · 2021Review
- Interrogating Plant-Microbe Interactions with Chemical Tools: Click Chemistry Reagents for Metabolic Labeling and Activity-Based Probes.Molecules (Basel, Switzerland) · 2021Review
- Metabolic labeling of glycerophospholipids via clickable analogs derivatized at the lipid headgroup.Chemistry and physics of lipids · 2020Review
- Metabolic phospholipid labeling of intact bacteria enables a fluorescence assay that detects compromised outer membranes.Journal of lipid research · 2020Article
- Greasing the Wheels of Lipid Biology with Chemical Tools.Trends in biochemical sciences · 2018Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPhospholipids are important structural and signaling molecules in plant membranes. Some fluorescent dyes can stain general lipids of membranes, but labeling and visualization of specific lipid classes have yet to be developed for most components of the membrane. New techniques for visualizing membrane lipids are needed to further delineate their dynamic structural and signaling roles in plant cells. In this study we examined whether propargylcholine, a bioortholog of choline, can be used to label the major membrane lipid, phosphatidylcholine, and other choline phospholipids in plants. We established that propargylcholine is readily taken up by roots, and that its incorporation is not detrimental to plant growth. After plant tissue is harvested and fixed, a click-chemistry reaction covalently links the alkyne group of propargylcholine to a fluorescently-tagged azide, resulting in specific labeling of choline phospholipids.
resultsUptake of propargylcholine, followed by click chemistry with fluorescein or Alexa Fluor 594 azide was used to visualize choline phospholipids in cells of root, leaf, stem, silique and seed tissues from
conclusionWe developed and validated a click-chemistry based method for fluorescence imaging of choline phospholipids using a bioortholog of choline, propargylcholine, in various cell-types and tissues from
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.