ArticleFrontiers in microbiology2018
Microbiome of Total Versus Live Bacteria in the Gut of Rex Rabbits.
Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Effect of rabbit gastrointestinal stasis (RGIS) on the fecal microbiota of pet rabbits (Oryctolagus cuniculus).PloS one · 2025Article
- Lactic acid bacteria reduce bacterial diarrhea in rabbits via enhancing immune function and restoring intestinal microbiota homeostasis.BMC veterinary research · 2024Article
- Crosstalk between gut microbiota and host immune system and its response to traumatic injury.Frontiers in immunology · 2024Review
- DNA from non-viable bacteria biases diversity estimates in the corals Acropora loripes and Pocillopora acuta.Environmental microbiome · 2023Article
- Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit Breeds.Animals : an open access journal from MDPI · 2023Article
- Normal bacterial flora of the oral cavity in healthy pet rabbits (Oryctolagus cuniculus).Veterinary medicine and science · 2023Article
- Altering Microbiomes with Hydroxyapatite Nanoparticles: A Metagenomic Analysis.Materials (Basel, Switzerland) · 2022Article
- Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine.Scientific reports · 2022Article
- Could Dietary Supplementation with Different Sources of N-3 Polyunsaturated Fatty Acids Modify the Rabbit Gut Microbiota?Antibiotics (Basel, Switzerland) · 2022Article
- Multifunctional carbonized nanogels to treat lethal acute hepatopancreatic necrosis disease.Journal of nanobiotechnology · 2021Article
- Rabbit microbiota across the whole body revealed by 16S rRNA gene amplicon sequencing.BMC microbiology · 2021Article
- Dead or alive: microbial viability treatment reveals both active and inactive bacterial constituents in the fish gut microbiota.Journal of applied microbiology · 2021Article
- Polyphenols: From Theory to Practice.Foods (Basel, Switzerland) · 2021Review
- Article
- Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA.Microbiome · 2021Article
- Review
- A muramidase from Acremonium alcalophilum hydrolyse peptidoglycan found in the gastrointestinal tract of broiler chickens.Journal of industrial microbiology & biotechnology · 2021Article
- Characterization of Bacterial Microbiota Composition along the Gastrointestinal Tract in Rabbits.Animals : an open access journal from MDPI · 2020Article
- Mechanistic insight into the gut microbiome and its interaction with host immunity and inflammation.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2020Review
- Transcriptome Analysis of the Effects of Fasting Caecotrophy on Hepatic Lipid Metabolism in New Zealand Rabbits.Animals : an open access journal from MDPI · 2019Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gastrointestinal bacteria are essential for host health, and only viable microorganisms contribute to gastrointestinal functions. When evaluating the gut microbiota by next generation sequencing method, dead bacteria, which compose a proportion of gut bacteria, may distort analysis of the live gut microbiota. We collected stomach, jejunum, ileum, cecum, and colon contents from Rex rabbits. A modified propidium monoazide (PMA) treatment protocol was used to exclude DNA from dead bacteria. Analysis of untreated samples yielded total bacteria, and analysis of PMA-treated samples yielded live bacteria. Quantitative polymerase chain reaction and 16S rRNA gene sequencing were performed to evaluate the live-to-total bacteria ratio and compare the difference between live and total microbiota in the entire digestive tract. A low proportion of live bacteria in the foregut (stomach 1.12%, jejunum 1.2%, ileum 2.84%) and a high proportion of live bacteria in the hindgut (cecum 24.66%, colon 19.08%) were observed. A significant difference existed between total and live microbiota.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.