ArticleChemistry Central journal2018
The determination of Ochratoxin A based on the electrochemical aptasensor by carbon aerogels and methylene blue assisted signal amplification.
Article in Chemistry Central journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 22 citations in OpenAlex.
- Aspergillus Mycotoxins: The Major Food Contaminants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Trends on Aerogel-Based Biosensors for Medical Applications: An Overview.International journal of molecular sciences · 2024Review
- Nanomaterial-based aptamer biosensors for ochratoxin A detection: a review.Analytical and bioanalytical chemistry · 2022Review
- Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring.Sensors (Basel, Switzerland) · 2020Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In this work, a novel aptamer-based electrochemical biosensor was developed for the determination of Ochratoxin A (OTA) by using carbon aerogels (CAs) and methylene blue (MB) as signal amplification strategy. CAs was used as carrier to load the abundant of complementary DNA (cDNA), which could enhance the hybridization between CAs-cDNA and aptamer immobilized on the electrode surface, thus provide more double-stranded DNA for MB intercalation. The current of MB on the CAs-cDNA/apt/AuE sensor was twice that on the cDNA/apt/AuE sensor, which indicated that the CAs with high surface area enabled a higher loading of the cDNA and absorbed more MB, thus realized the signal amplification strategy. The optimum experimental conditions including MB incubation time of 15 min, aptamer concentration of 4.0 μmol/L, hybridization time of 2.0 h, and OTA incubation time of 18 min were obtained. The change of peak current was linearly proportional to the OTA concentration in the range of 0.10-10 ng/mL with the actual detection limit of 1.0 × 10
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