ArticleEmerging microbes & infections2018
A novel SigB(Q225P) mutation in Staphylococcus aureus retains virulence but promotes biofilm formation.
Article in Emerging microbes & infections, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 35 citations in OpenAlex.
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- Article
- Large-scale genetic analysis and biological traits of two SigB factors inFrontiers in microbiology · 2023Article
- Loaded delta-hemolysin shapes the properties ofFrontiers in microbiology · 2023Article
- A vancomycin resistance-associated WalK(S221P) mutation attenuates the virulence of vancomycin-intermediate Staphylococcus aureus.Journal of advanced research · 2022Article
- Antibiotics Resistance and Virulence ofFoods (Basel, Switzerland) · 2022Article
- The Q225P Mutation in SigB Promotes Membrane Vesicle Formation in Staphylococcus aureus.Current microbiology · 2022Article
- Accessory Gene Regulator (Frontiers in microbiology · 2022Article
- Genetic and compositional analysis of biofilm formed byFrontiers in microbiology · 2022Article
- Acapsular Staphylococcus aureus with a non-functional agr regains capsule expression after passage through the bloodstream in a bacteremia mouse model.Scientific reports · 2020Article
- Investigation of biofilm production and its association with genetic and phenotypic characteristics of OM (osteomyelitis) and non-OM orthopedic Staphylococcus aureus.Annals of clinical microbiology and antimicrobials · 2020Article
- Fighting Mixed-Species Microbial Biofilms With Cold Atmospheric Plasma.Frontiers in microbiology · 2020Review
- Advanced Glycation End Products Enhance Biofilm Formation by Promoting Extracellular DNA Release ThroughFrontiers in microbiology · 2020Article
- Within-host evolution of bovine Staphylococcus aureus selects for a SigB-deficient pathotype characterized by reduced virulence but enhanced proteolytic activity and biofilm formation.Scientific reports · 2019Article
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Authors and funding
16 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureus is an important pathogen that produces abundant virulence factors, which cause various diseases that burden human health worldwide. The stress response regulon called sigma factor B (SigB) is a well-characterized global regulator that is involved in the regulation of S. aureus virulence, pigmentation, and biofilm formation. However, the regulatory network upon SigB in S. aureus is incompletely described. Here, we identified a novel substitution mutation, SigB(Q225P), which contributed the nonpigmented phenotype of S. aureus. The S. aureus mutant carrying SigB(Q225P) substitution lacks staphyloxanthin, a key virulence factor in protecting bacteria from host-oxidant killing, but retains bacterial pathogenicity with pleiotropic alterations in virulence factors, resulting in similar lethality and abscess formation ability in animal models. We also reported the SigB(Q225P) promotion of biofilm formation in S. aureus. Real-time quantitative polymerase chain reaction (RT-qPCR) revealed that the expression of nuc gene, which encodes thermonuclease, was significantly downregulated, resulting in accumulation of eDNA in the biofilm of SigB(Q225P) mutant strain. LacZ reporter assay showed that SigB(Q225P) influenced the activity of nuc promoter. Furthermore, electrophoretic mobility shift assay (EMSA) and Bio-layer interferometry (BLI) assay revealed that both SigB and SigB(Q225P) proteins could directly bind to nuc gene promoter; however, the binding activity decreased for SigB(Q225P). Our data renewed the understanding of the relationship between S. aureus golden pigment and its virulence and suggested that a single substitution mutation in SigB might enhance the biofilm formation of S. aureus by directly downregulating nuc expression.
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