Evidence map›Paper›PMID 29689462›Full record

ArticleVirology2018

Macaque homologs of Kaposi's sarcoma-associated herpesvirus (KSHV) infect germinal center lymphoid cells, epithelial cells in skin and gastrointestinal tract and gonadal germ cells in naturally infected macaques.

Helle Bielefeldt-Ohmann, A Gregory Bruce, Kellie Howard, Minako Ikoma, Margaret E Thouless, Timothy M Rose

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Helle Bielefeldt-OhmannWashington National Primate Research Center, University of Washington, Seattle, WA, USA. Electronic address: h.bielefeldtohmann1@uq.edu.au.
A Gregory BruceCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA; Department of Pathobiology, University of Washington, Seattle, WA, USA. Electronic address: agregbruce@gmail.com.
Kellie HowardCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA; Department of Pathobiology, University of Washington, Seattle, WA, USA. Electronic address: khoward@adaptivebiotech.com.
Minako IkomaCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA. Electronic address: minako.oda@seattlechildrens.org.
Margaret E ThoulessDepartment of Pathobiology, University of Washington, Seattle, WA, USA. Electronic address: methoul@uw.edu.
Timothy M RoseCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA; Department of Pathobiology, University of Washington, Seattle, WA, USA; Department of Pediatrics, University of Washington, Seattle, WA, USA. Electronic address: timothy.rose@seattlechildrens.org.
Center for Infectious Disease Research · USUniversity of Washington · US

Funding

Herpesvirus latency and reactivation in macaque models of human diseaseR24RR023343 · NCRR · UNIVERSITY OF WASHINGTON · PI ROSE, TIMOTHY M · 2007 to 2011
$3.2M
NCRR NIH HHS R24 RR023343
6 · The paper itself

Abstract

We developed a set of rabbit antisera to characterize infections by the macaque RV2 rhadinovirus homologs of KSHV. We analyzed tissues from rhesus and pig-tailed macaques naturally infected with rhesus rhadinovirus (RRV) or Macaca nemestrina rhadinovirus 2 (MneRV2). Our study demonstrates that RV2 rhadinoviruses have a tropism for epithelial cells, lymphocytes and gonadal germ cells in vivo. We observed latent infections in both undifferentiated and differentiated epithelial cells with expression of the latency marker, LANA. Expression of the early (ORF59) and late (glycoprotein B) lytic markers were detected in highly differentiated cells in epithelial ducts in oral, renal, dermal and gastric mucosal tissue as well as differentiated germ cells in male and female gonads. Our data provides evidence that epithelial and germ cell differentiation in vivo induces rhadinovirus reactivation and suggests that infected epithelial and germ cells play a role in transmission and dissemination of RV2 rhadinovirus infections in vivo.

Indexed as

AnimalsAntigens, ViralEpithelial CellsGastrointestinal TractGerm CellsGerminal CenterGonadsHerpesviridae InfectionsHerpesvirus 8, HumanImmunity, InnateLymphocytesMacaca mulattaMacaca nemestrinaNuclear ProteinsRabbitsRhadinovirusAntigens, Virallatency-associated nuclear antigenNuclear ProteinsSA8 glycoprotein B, simian herpesvirusViral Envelope ProteinsViral ProteinsEpithelialGerm cellImmunohistochemistryKSHVLymphocyteMacaqueMneRV2RhadinovirusRRV

Identifiers

PMID29689462
PMCPMC5971157
OpenAlexW2799890318

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.