ArticlePloS one2018
Zebrafish-based identification of the antiseizure nucleoside inosine from the marine diatom Skeletonema marinoi.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
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- Marine Natural Products Rescuing the Eye: A Narrative Review.Marine drugs · 2024Review
- Animal models of silicosis: fishing for new therapeutic targets and treatments.European respiratory review : an official journal of the European Respiratory Society · 2023Review
- Zebrafish models of COVID-19.FEMS microbiology reviews · 2023Review
- The MicroalgaMolecules (Basel, Switzerland) · 2022Article
- From the Sea for the Sight: Marine Derived Products for Human Vision.Frontiers in aging neuroscience · 2022Review
- Editorial of Special Issue "Microalgal Molecules and Enzymes".International journal of molecular sciences · 2021Article
- First Report of OvoA Gene in Marine Arthropods: A New Candidate Stress Biomarker in Copepods.Marine drugs · 2021Article
- Unlocking the Health Potential of Microalgae as Sustainable Sources of Bioactive Compounds.International journal of molecular sciences · 2021Review
- Potential ApproachesFrontiers in oncology · 2021Review
- Inosine as a Tool to Understand and Treat Central Nervous System Disorders: A Neglected Actor?Frontiers in neuroscience · 2021Review
- Chemical Defense in Marine Organisms.Marine drugs · 2020Article
- De novo Transcriptome of the Non-saxitoxin ProducingMarine drugs · 2020Article
- Ten-Year Research Update Review: Antiviral Activities from Marine Organisms.Biomolecules · 2020Review
- A Proline Derivative-Enriched Fraction fromInternational journal of molecular sciences · 2020Article
- In Silico Identification of Type III PKS Chalcone and Stilbene Synthase Homologs in Marine Photosynthetic Organisms.Biology · 2020Article
- The Influence of Palmatine Isolated fromCells · 2020Article
- PheophorbideMarine drugs · 2020Review
- Monogalactosyldiacylglycerol and Sulfolipid Synthesis in Microalgae.Marine drugs · 2020Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the goal of identifying neuroactive secondary metabolites from microalgae, a microscale in vivo zebrafish bioassay for antiseizure activity was used to evaluate bioactivities of the diatom Skeletonema marinoi, which was recently revealed as being a promising source of drug-like small molecules. A freeze-dried culture of S. marinoi was extracted by solvents with increasing polarities (hexane, dichloromethane, methanol and water) and these extracts were screened for anticonvulsant activity using a larval zebrafish epilepsy model with seizures induced by the GABAA antagonist pentylenetetrazole. The methanolic extract of S. marinoi exhibited significant anticonvulsant activity and was chosen for bioassay-guided fractionation, which associated the bioactivity with minor constituents. The key anticonvulsant constituent was identified as the nucleoside inosine, a well-known adenosine receptor agonist with previously reported antiseizure activities in mice and rat epilepsy models, but not reported to date as a bioactive constituent of microalgae. In addition, a UHPLC-HRMS metabolite profiling was used for dereplication of the other constituents of S. marinoi. Structures of the isolated compounds were elucidated by nuclear magnetic resonance and high-resolution spectrometry. These results highlight the potential of zebrafish-based screening and bioassay-guided fractionation to identify neuroactive marine natural products.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.