Cell regeneration and cyclic catalysis of engineered Kluyveromyces marxianus of a D-psicose-3-epimerase gene from Agrobacterium tumefaciens for D-allulose production.

Peizhou Yang, Xingxing Zhu, Zhi Zheng, Dongdong Mu, Shaotong Jiang, Shuizhong Luo, Yun Wu, Minrui Du

Abstract read

In one paragraph

Article in World journal of microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it

0cells of the map it votes in

6citing papers in PubMed

1.5field-weighted citation impact, top 17% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 20 citations in OpenAlex.

Recent Advances in the Biosynthesis of Natural Sugar Substitutes in Yeast.Journal of fungi (Basel, Switzerland) · 2023
Review
RNA polymerase II-driven CRISPR-Cas9 system for efficient non-growth-biased metabolic engineering ofMetabolic engineering communications · 2022
Article
Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes.Foods (Basel, Switzerland) · 2021
Review
Review onFrontiers in bioengineering and biotechnology · 2020
Review
High Conversion of D-Fructose into D-Allulose by Enzymes Coupling with an ATP Regeneration System.Molecular biotechnology · 2019
Article
Biochemical characterization and biocatalytic application of a novel d-tagatose 3-epimerase fromRSC advances · 2019
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Peizhou YangCollege of Food Science and Engineering, Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, Anhui, China. yangpeizhou@163.com.

Xingxing ZhuCollege of Food Science and Engineering, Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, Anhui, China.

Zhi ZhengCollege of Food Science and Engineering, Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, Anhui, China.

Dongdong MuCollege of Food Science and Engineering, Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, Anhui, China.

Shaotong JiangCollege of Food Science and Engineering, Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, Anhui, China.

Shuizhong LuoCollege of Food Science and Engineering, Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, Anhui, China.

Yun WuCollege of Food Science and Engineering, Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, Anhui, China.

Minrui DuCollege of Food Science and Engineering, Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, Anhui, China.

Hefei University of Technology · CN

Funding

Key Science and Technology Program of Anhui Province No.1604a0702001

6 · The paper itself

Abstract

D-Allulose as a low-energy and special bioactive monosaccharide sugar is essential for human health. In this study, the D-psicose-3-epimerase gene (DPEase) of Agrobacterium tumefaciens was transferred into thermotolerant Kluyveromyces marxianus to decrease the production cost of D-allulose and reduce the number of manufacturing procedures. The cell regeneration of K. marxianus and cyclic catalysis via whole-cell reaction were investigated to achieve the sustainable application of K. marxianus and the consumption of residual D-fructose. Results showed that DPEase, encoding a 33 kDa protein, could be effectively expressed in thermotolerant K. marxianus. The engineered K. marxianus produced 190 g L

Indexed as

RegenerationAgrobacterium tumefaciensCarbohydrate EpimerasesCatalysisCloning, MolecularEnzyme StabilityEthanolFermentationFructoseGene Expression Regulation, EnzymologicGenetic VectorsHydrogen-Ion ConcentrationKluyveromycesMetabolic EngineeringTemperatureTime FactorsCarbohydrate EpimerasesEthanolFructosepsicoseAgrobacterium tumefaciensD-AlluloseD-Psicose-3-epimeraseKluyveromyces marxianusThermotolerant

Identifiers

PMID29687334

DOI10.1007/s11274-018-2451-6

OpenAlexW2799632166

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.