Evidence map›Paper›PMID 29687245›Full record

ArticleJournal of plant research2018

Development and characterization of 12 polymorphic microsatellite loci in the sea sandwort, Honckenya peploides.

Meg C Gravley, George K Sage, Sandra L Talbot, Matthew L Carlson

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Article in Journal of plant research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Meg C GravleyU.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA. mgravley@usgs.gov.
George K SageU.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA.
Sandra L TalbotU.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA.
Matthew L CarlsonBiological Sciences Department, University of Alaska Anchorage, 3211 Providence Drive, Anchorage, AK, 99508, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Codominant marker systems are better suited to analyze population structure and assess the source of an individual in admixture analyses. Currently, there is no codominant marker system using microsatellites developed for the sea sandwort, Honckenya peploides (L.) Ehrh., an early colonizer in island systems. We developed and characterized novel microsatellite loci from H. peploides, using reads collected from whole genome shotgun sequencing on a 454 platform. The combined output from two shotgun runs yielded a total of 62,669 reads, from which 58 loci were screened. We identified 12 polymorphic loci that amplified reliably and exhibited disomic inheritance. Microsatellite data were collected and characterized for the 12 polymorphic loci in two Alaskan populations of H. peploides: Fossil Beach, Kodiak Island (n = 32) and Egg Bay, Atka Island (n = 29). The Atka population exhibited a slightly higher average number of alleles (3.9) and observed heterozygosity (0.483) than the Kodiak population (3.3 and 0.347, respectively). The overall probability of identity values for both populations was PID = 2.892e

Indexed as

AllelesCaryophyllaceaeGenetic LociHeterozygoteHigh-Throughput Nucleotide SequencingMicrosatellite RepeatsPolymorphism, GeneticDisomic inheritanceHonckenya peploidesMicrosatellitesNext-generation sequencingSea sandwort

Identifiers

PMID29687245

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.