Evidence map›Paper›PMID 29687223›Full record

ArticlePlanta2018

Development of glyphosate-resistant alfalfa (Medicago sativa L.) upon transformation with the GR79Ms gene encoding 5-enolpyruvylshikimate-3-phosphate synthase.

Dengxia Yi, Lin Ma, Min Lin, Cong Li

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Co-expression ofaBIOTECH · 2023
    Article
  3. Review
  4. Review
  5. Transgenic Cotton (Frontiers in plant science · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Dengxia YiInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Lin MaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Min LinBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Cong LiInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. licong05200@163.com.
Chinese Academy of Agricultural Sciences · CNBiotechnology Research Institute · CN

Funding

Beijing Natural Science Foundation 6184047the Agricultural Science and Technology Innovation Program of China ASTIP-IAS10the Fundamental Research Funds for Central Non-profit Scientific Institution 2016ywf-yb-10the Fundamental Research Funds for Central Non-profit Scientific Institution 2018-YWF-YB-4the Modern Agricultural Industry Technology Research System CARS34the National Natural Science Foundation of China 31372362
6 · The paper itself

Abstract

MAIN

conclusionThe glyphosate-resistant gene, GR79Ms, was successfully introduced into the genome of alfalfa. The transgenic events may serve as novel germplasm resources in alfalfa breeding. Weed competition can reduce the alfalfa yield, generating new alfalfa germplasm with herbicide resistance is essential. To obtain transgenic alfalfa lines with glyphosate resistance, a new synthetic glyphosate-resistant gene GR79Ms encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was introduced into alfalfa germplasm by Agrobacterium tumefaciens-mediated transformation. In total, 67 transformants were obtained. PCR and Southern blot analyses confirmed that GR79Ms was successfully inserted into the genome of alfalfa. Reverse transcription-PCR and western blot analyses further demonstrated the expression of GR79Ms and its product, GR79Ms EPSPS. Moreover, two homozygous transgenic lines were developed in the T

Indexed as

Herbicides3-Phosphoshikimate 1-CarboxyvinyltransferaseBlotting, SouthernBlotting, WesternGlycineGlyphosateHerbicide ResistanceMedicago sativaPlants, Genetically ModifiedReverse Transcriptase Polymerase Chain Reaction3-Phosphoshikimate 1-CarboxyvinyltransferaseGlycineGlyphosateHerbicidesAgrobacterium tumefaciens-mediated transformationGlyphosate toleranceGR79MsMolecular analysisTransgenic alfalfa

Identifiers

PMID29687223
OpenAlexW2799298922

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.