ArticleScientific reports2018
Electrochemical affinity biosensors for fast detection of gene-specific methylations with no need for bisulfite and amplification treatments.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 79 citations in OpenAlex.
- Tracking Globally 5-Methylcytosine and Its Oxidized Derivatives in Colorectal Cancer Epigenome Using Bioelectroanalytical Technologies.ACS sensors · 2025Article
- Perspectives on the Application of Biosensors for the Early Detection of Oral Cancer.Sensors (Basel, Switzerland) · 2025Review
- Recent advances in ctDNA detection using electrochemical biosensor for cancer.Discover oncology · 2024Review
- Recent Advancements in the Application of Circulating Tumor DNA as Biomarkers for Early Detection of Cancers.ACS biomaterials science & engineering · 2024Review
- Emerging ctDNA detection strategies in clinical cancer theranostics.Smart medicine · 2023Review
- Antibody-Loaded Nanoplatforms for Colorectal Cancer Diagnosis and Treatment: An Update.Pharmaceutics · 2023Review
- Hybridization-based CpG methylation level detection using methyl-CpG-binding domain-fused luciferase.Analytical and bioanalytical chemistry · 2023Article
- Recent Progress in Nanotechnology-Based Approaches for Food Monitoring.Nanomaterials (Basel, Switzerland) · 2022Review
- Electrochemical Biosensors for Circulating Tumor DNA Detection.Biosensors · 2022Review
- Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation.Biosensors · 2022Article
- Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor.Nature biomedical engineering · 2021Article
- 2D materials as a diagnostic platform for the detection and sensing of the SARS-CoV-2 virus: a bird's-eye view.Journal of materials chemistry. B · 2021Review
- Self-Assembled Monolayers: Versatile Uses in Electronic Devices from Gate Dielectrics, Dopants, and Biosensing Linkers.Micromachines · 2021Review
- A polyaniline/platinum coated fiber optic surface plasmon resonance sensor for picomolar detection of 4-nitrophenol.Scientific reports · 2021Article
- DNA Methylation in Solid Tumors: Functions and Methods of Detection.International journal of molecular sciences · 2021Review
- A Label-Free DNA-Immunosensor Based on Aminated rGO Electrode for the Quantification of DNA Methylation.Nanomaterials (Basel, Switzerland) · 2021Article
- Non-Invasive Electrochemical Biosensors for TNF-α Cytokines Detection in Body Fluids.Frontiers in bioengineering and biotechnology · 2021Review
- Electrochemically detecting DNA methylation in the EN1 gene promoter: implications for understanding ageing and disease.Bioscience reports · 2020Article
- Multiprobe Assay for ClinicalACS omega · 2020Article
- Application of magnetic nanoparticles in nucleic acid detection.Journal of nanobiotechnology · 2020Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper describes two different electrochemical affinity biosensing approaches for the simple, fast and bisulfite and PCR-free quantification of 5-methylated cytosines (5-mC) in DNA using the anti-5-mC antibody as biorecognition element. One of the biosensing approaches used the anti-5-mC as capture bioreceptor and a sandwich type immunoassay, while the other one involved the use of a specific DNA probe and the anti-5-mC as a detector bioreceptor of the captured methylated DNA. Both strategies, named for simplicity in the text as immunosensor and DNA sensor, respectively, were implemented on the surface of magnetic microparticles and the transduction was accomplished by amperometry at screen-printed carbon electrodes by means of the hydrogen peroxide/hydroquinone system. The resulting amperometric biosensors demonstrated reproducibility throughout the entire protocol, sensitive determination with no need for using amplification strategies, and competitiveness with the conventional enzyme-linked immunosorbent assay methodology and the few electrochemical biosensors reported so far in terms of simplicity, sensitivity and assay time. The DNA sensor exhibited higher sensitivity and allowed the detection of the gene-specific methylations conversely to the immunosensor, which detected global DNA methylation. In addition, the DNA sensor demonstrated successful applicability for 1 h-analysis of specific methylation in two relevant tumor suppressor genes in spiked biological fluids and in genomic DNA extracted from human glioblastoma cells.
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