Evidence map›Paper›PMID 29686270›Full record

ArticleScientific reports2018

Spatial patterning of the Notch ligand Dll4 controls endothelial sprouting in vitro.

L A Tiemeijer, J-P Frimat, O M J A Stassen, C V C Bouten, C M Sahlgren

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 18 citations in OpenAlex.

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  8. Engineering a collagen matrix for cell-instructive regenerative angiogenesis.Journal of biomedical materials research. Part B, Applied biomaterials · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

L A TiemeijerDepartment of Biomedical Engineering, Soft Tissue Engineering and Mechanobiology, ICMS Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, Netherlands. l.a.tiemeijer@tue.nl.
J-P FrimatDepartment of Mechanical Engineering, Microsystems Group, Eindhoven University of Technology, Eindhoven, Netherlands.
O M J A StassenDepartment of Biomedical Engineering, Soft Tissue Engineering and Mechanobiology, ICMS Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, Netherlands.
C V C BoutenDepartment of Biomedical Engineering, Soft Tissue Engineering and Mechanobiology, ICMS Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, Netherlands.
C M SahlgrenDepartment of Biomedical Engineering, Soft Tissue Engineering and Mechanobiology, ICMS Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, Netherlands. c.m.sahlgren@tue.nl.
Eindhoven University of Technology · NLÅbo Akademi University · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Angiogenesis, the formation of new blood vessels, is a vital process for tissue growth and development. The Notch cell-cell signalling pathway plays an important role in endothelial cell specification during angiogenesis. Dll4 - Notch1 signalling directs endothelial cells into migrating tip or proliferating stalk cells. We used the directing properties of Dll4 to spatially control endothelial cell fate and the direction of endothelial sprouts. We created linear arrays of immobilized Dll4 using micro contact printing. HUVECs were seeded perpendicular to these Dll4 patterns using removable microfluidic channels. The Notch activating properties of surface immobilized Dll4 were confirmed by qPCR. After induction of sprouting, microscopic images of fluorescently labelled endothelial sprouts were analysed to determine the direction and the efficiency of controlled sprouting (Ecs). Directionality analysis of the sprouts showed the Dll4 pattern changes sprout direction from random to unidirectional. This was confirmed by the increase of Ecs from 54.5 ± 3.1% for the control, to an average of 84.7 ± 1.86% on the Dll4 patterned surfaces. Our data demonstrates a surface-based method to spatially pattern Dll4 to gain control over endothelial sprout location and direction. This suggests that spatial ligand patterning can be used to provide control over (neo) vascularization.

Indexed as

Adaptor Proteins, Signal TransducingCalcium-Binding ProteinsEndothelium, VascularHumansHuman Umbilical Vein Endothelial CellsIntercellular Signaling Peptides and ProteinsLigandsMicrofluidicsNeovascularization, PhysiologicSignal TransductionAdaptor Proteins, Signal TransducingCalcium-Binding ProteinsDLL4 protein, humanIntercellular Signaling Peptides and ProteinsLigands

Identifiers

PMID29686270
PMCPMC5913301
OpenAlexW2803092362

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.