ArticleParasites & vectors2018
Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- A global systematic review and meta-analysis on the babesiosis in dogs with special reference to Babesia canis.Veterinary medicine and science · 2024Pooled it
- Transfection ofBio-protocol · 2024Article
- Establishment of ContinuousMicrobiology spectrum · 2023Article
- Establishment of a stable transfection and gene targeting system inFrontiers in cellular and infection microbiology · 2023Article
- Establishment of a Transient and Stable Transfection System forFrontiers in cellular and infection microbiology · 2022Article
- Recent Advances in Molecular Genetic Tools forVeterinary sciences · 2021Review
- Stable transfection system for Babesia sp. Xinjiang.Parasites & vectors · 2021Article
- A framework for signaling throughout the life cycle of Babesia species.Molecular microbiology · 2021Review
- Development of a stable transgenic Theileria equi parasite expressing an enhanced green fluorescent protein/blasticidin S deaminase.Scientific reports · 2021Article
- Establishment of a stable transfection method in Babesia microti and identification of a novel bidirectional promoter of Babesia microti.Scientific reports · 2020Article
- Transient Transfection of the Zoonotic ParasitePathogens (Basel, Switzerland) · 2020Article
- Genome Editing ofmSphere · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundGenetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis.
resultsGFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis.
conclusionsWe present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.