ArticleVirology journal2018
Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future.
Article in Virology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Development of an immunochromatographic lateral flow assay for the rapid detection of all known orthoebolavirus glycoproteins.Npj viruses · 2026Article
- A snapshot on molecular technologies for diagnosing FAdV infections.Frontiers in veterinary science · 2025Review
- Application of recombinase polymerase amplification with lateral flow assay to pathogen point-of-care diagnosis.Frontiers in cellular and infection microbiology · 2024Review
- Rapid, sensitive, and specific, low-resource molecular detection of Hendra virus.One health (Amsterdam, Netherlands) · 2023Article
- Rapid Diagnostic Tests for the Detection of the Four Dengue Virus Serotypes in Clinically Relevant Matrices.Microbiology spectrum · 2023Article
- Recombinase Polymerase Amplification-Based Biosensors for Rapid Zoonoses Screening.International journal of nanomedicine · 2023Review
- A Systematic Review on Suitability of Molecular Techniques for Diagnosis and Research into Infectious Diseases of Concern in Resource-Limited Settings.Current issues in molecular biology · 2022Review
- Fluorescent Biosensors for the Detection of Viruses Using Graphene and Two-Dimensional Carbon Nanomaterials.Biosensors · 2022Review
- Evaluation of three rapid low-resource molecular tests for Nipah virus.Frontiers in microbiology · 2022Article
- Rapid molecular assays for the detection of the four dengue viruses in infected mosquitoes.Gates open research · 2022Article
- Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow-based detection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Tracking SARS-CoV-2: Novel Trends and Diagnostic Strategies.Diagnostics (Basel, Switzerland) · 2021Review
- Antagonism of STAT3 signalling by Ebola virus.PLoS pathogens · 2021Article
- Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.Biosensors · 2021Article
- Ebola Virus Disease and Current Therapeutic Strategies: A Review.Advances in experimental medicine and biology · 2021Review
- A novel recombinase polymerase amplification assay for rapid detection of epidemic fowl adenovirus.Poultry science · 2020Article
- A flexible format LAMP assay for rapid detection of Ebola virus.PLoS neglected tropical diseases · 2020Article
- COVID-19 Infection Diagnosis: Potential Impact of Isothermal Amplification Technology to Reduce Community Transmission of SARS-CoV-2.Diagnostics (Basel, Switzerland) · 2020Review
- Label-Free Pathogen Detection by a Deoxyribozyme Cascade with Visual Signal Readout.Sensors and actuators. B, Chemical · 2019Article
- Optofluidic Amplification-free Multiplex Detection of Viral Hemorrhagic Fevers.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe 2014/2015 Ebolavirus outbreak resulted in more than 28,000 cases and 11,323 reported deaths, as of March 2016. Domestic transmission of the Guinea strain associated with the outbreak occurred mainly in six African countries, and international transmission was reported in four countries. Outbreak management was limited by the inability to rapidly diagnose infected cases. A further fifteen countries in Africa are predicted to be at risk of Ebolavirus outbreaks in the future as a consequence of climate change and urbanization. Early detection of cases and reduction of transmission rates is critical to prevent and manage future severe outbreaks. We designed a rapid assay for detection of Ebolavirus using recombinase polymerase amplification, a rapid isothermal amplification technology that can be combined with portable lateral flow detection technology. The developed rapid assay operates in 30 min and was comparable with real-time TaqMan™ PCR.
methodsDesigned, screened, selected and optimized oligonucleotides using the NP coding region from Ebola Zaire virus (Guinea strain). We determined the analytical sensitivity of our Ebola rapid molecular test by testing selected primers and probe with tenfold serial dilutions (1.34 × 10
resultsThe assay had a detection limit of 134 copies per μL of plasmid containing the NP gene of Ebolavirus Mayinga, and cultured Ebolavirus and was highly specific for the Zaire ebolavirus species, including the Guinea strain responsible for the 2014/2015 outbreak. The assay did not detect related viruses like Marburg, Reston, or Sudan viruses, and other pathogens likely to be isolated from clinical samples.
conclusionsOur assay could be suitable for implementation in district and primary health laboratories, as only a heating block and centrifuge is required for operation. The technique could provide a pathway for rapid screening of patients and animals for improved management of outbreaks.
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