ArticleBiochimica et biophysica acta. Biomembranes2018
Effects of alterations of the E. coli lipopolysaccharide layer on membrane permeabilization events induced by Cecropin A.
Article in Biochimica et biophysica acta. Biomembranes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- AApplied and environmental microbiology · 2026Article
- Membrane-Traversing Peptides: Mechanistic Landscapes, Assays, and Applications.Chemical reviews · 2026Review
- Microbiome features associated with persistent intestinal carriages of Escherichia coli ST131 in a Southeast Asian cohort study.Nature communications · 2026Article
- AFrontiers in microbiology · 2026Article
- IITR00803: a benzoxazole-nitrothiophene small molecule with broad-spectrum antibacterial potential.Microbiology spectrum · 2025Article
- Multiple resistance factors collectively promote inoculum-dependent dynamic survival during antimicrobial peptide exposure in Enterobacter cloacae.PLoS pathogens · 2024Article
- Multiple resistance factors collectively promote inoculum-dependent dynamic survival during antimicrobial peptide exposure inbioRxiv : the preprint server for biology · 2024Article
- Real-Time Fluorescence Microscopy on LivingInternational journal of molecular sciences · 2022Article
- A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli.Bioresources and bioprocessing · 2021Article
- Article
- Long-term effects of the proline-rich antimicrobial peptide Oncocin112 on theThe Journal of biological chemistry · 2020Article
- Application of Biophysical Techniques to Investigate the Interaction of Antimicrobial Peptides With Bacterial Cells.Frontiers in medical technology · 2020Review
- Resistance of early stationary phase E. coli to membrane permeabilization by the antimicrobial peptide Cecropin A.Biochimica et biophysica acta. Biomembranes · 2019Article
- Article
- Top-Down Characterization of Lipooligosaccharides from Antibiotic-Resistant Bacteria.Analytical chemistry · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The outermost layer of Gram negative bacteria is composed of a lipopolysaccharide (LPS) network that forms a dense protective hydrophilic barrier against entry of hydrophobic drugs. At low μM concentrations, a large family of cationic polypeptides known as antimicrobial peptides (AMPs) are able to penetrate the LPS layer and permeabilize the outer membrane (OM) and the cytoplasmic membrane (CM), causing cell death. Cecropin A is a well-studied cationic AMP from moth. Here a battery of time-resolved, single-cell microscopy experiments explores how deletion of sugar layers and/or phosphoryl negative charges from the core oligosaccharide layer (core OS) of K12 E. coli alters the timing of OM and CM permeabilization induced by Cecropin A. Deletion of sugar layers, or phosphoryl charges, or both from the core OS shortens the time to the onset of OM permeabilization to periplasmic GFP and also the lag time between OM permeabilization and CM permeabilization. Meanwhile, the 12-h minimum inhibitory concentration (MIC) changes only twofold with core OS alterations. The results suggest a two-step model in which the core oligosaccharide layers act as a kinetic barrier to penetration of Cecropin A to the lipid A outer leaflet of the OM. Once a threshold concentration has built up at the lipid A leaflet, nucleation occurs and the OM is locally permeabilized to GFP and, by inference, to Cecropin A. Whenever Cecropin A permeabilizes the OM, CM permeabilization always follows, and cell growth subsequently halts and never recovers on the 45 min observation timescale.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.