ArticleNucleic acids research2018
The exon-intron gene structure upstream of the initiation codon predicts translation efficiency.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- From Structure to Function of Promoters and 5'UTRs in Maize.International journal of molecular sciences · 2026Review
- Integrated analysis of post-transcriptional regulations reveals insights into acute myeloid leukemia.Communications biology · 2025Article
- Bioinformatics frameworks for single-cell long-read sequencing: unlocking isoform-level resolution.Briefings in bioinformatics · 2025Review
- RIBOSS detects novel translational events by combining long- and short-read transcriptome and translatome profiling.Briefings in bioinformatics · 2025Article
- Translation of the downstream ORF from bicistronic mRNAs by human cells: Impact of codon usage and splicing in the upstream ORF.Protein science : a publication of the Protein Society · 2025Article
- Circular RNAs: New layer of complexity evading breast cancer heterogeneity.Non-coding RNA research · 2023Article
- Multifunctional RNA-binding proteins influence mRNA abundance and translational efficiency of distinct sets of target genes.PLoS computational biology · 2021Article
- Analysis of 11,430 recombinant protein production experiments reveals that protein yield is tunable by synonymous codon changes of translation initiation sites.PLoS computational biology · 2021Article
- Analysis of Fungal Genomes Reveals Commonalities of Intron Gain or Loss and Functions in Intron-Poor Species.Molecular biology and evolution · 2021Article
- TISIGNER.com: web services for improving recombinant protein production.Nucleic acids research · 2021Article
- CodAn: predictive models for precise identification of coding regions in eukaryotic transcripts.Briefings in bioinformatics · 2021Article
- Transcription initiation of distant core promoters in a large-sized genome of an insect.BMC biology · 2021Article
- 5' Untranslated Region Elements Show High Abundance and Great Variability in Homologous ABCA Subfamily Genes.International journal of molecular sciences · 2020Article
- Determinants of translation efficiency in the evolutionarily-divergent protist Trichomonas vaginalis.BMC molecular and cell biology · 2020Article
- First Come, First Served: Sui Generis Features of the First Intron.Plants (Basel, Switzerland) · 2020Review
- Alternative ORFs and small ORFs: shedding light on the dark proteome.Nucleic acids research · 2020Review
- uORF-mediated translational control: recently elucidated mechanisms and implications in cancer.RNA biology · 2019Review
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Introns in mRNA leaders are common in complex eukaryotes, but often overlooked. These introns are spliced out before translation, leaving exon-exon junctions in the mRNA leaders (leader EEJs). Our multi-omic approach shows that the number of leader EEJs inversely correlates with the main protein translation, as does the number of upstream open reading frames (uORFs). Across the five species studied, the lowest levels of translation were observed for mRNAs with both leader EEJs and uORFs (29%). This class of mRNAs also have ribosome footprints on uORFs, with strong triplet periodicity indicating uORF translation. Furthermore, the positions of both leader EEJ and uORF are conserved between human and mouse. Thus, the uORF, in combination with leader EEJ predicts lower expression for nearly one-third of eukaryotic proteins.
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