ArticlePLoS biology2018
In vivo insertion pool sequencing identifies virulence factors in a complex fungal-host interaction.
Article in PLoS biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Ustilago maydis Seedling Infection Assay and Scoring.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Induction of Ustilago maydis Appressorium and Filamentation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Next-Generation Sequencing Applications for the Study of Fungal Pathogens.Microorganisms · 2022Review
- Effector-mediated relocalization of a maize lipoxygenase protein triggers susceptibility to Ustilago maydis.The Plant cell · 2022Article
- High-throughput estimation of allele frequencies using combined pooled-population sequencing and haplotype-based data processing.Plant methods · 2022Article
- Analysis of Fungal Genomes Reveals Commonalities of Intron Gain or Loss and Functions in Intron-Poor Species.Molecular biology and evolution · 2021Article
- Developing Functional Genomics Platforms for Fungi.mSystems · 2021Article
- The Pleiades are a cluster of fungal effectors that inhibit host defenses.PLoS pathogens · 2021Article
- Identification and Characterization of Two Transmembrane Proteins Required for Virulence ofFrontiers in plant science · 2021Article
- Insertion Pool Sequencing for Insertional Mutant Analysis in Complex Host-Microbe Interactions.Current protocols in plant biology · 2019Article
- Systematic Y2H Screening Reveals Extensive Effector-Complex Formation.Frontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Large-scale insertional mutagenesis screens can be powerful genome-wide tools if they are streamlined with efficient downstream analysis, which is a serious bottleneck in complex biological systems. A major impediment to the success of next-generation sequencing (NGS)-based screens for virulence factors is that the genetic material of pathogens is often underrepresented within the eukaryotic host, making detection extremely challenging. We therefore established insertion Pool-Sequencing (iPool-Seq) on maize infected with the biotrophic fungus U. maydis. iPool-Seq features tagmentation, unique molecular barcodes, and affinity purification of pathogen insertion mutant DNA from in vivo-infected tissues. In a proof of concept using iPool-Seq, we identified 28 virulence factors, including 23 that were previously uncharacterized, from an initial pool of 195 candidate effector mutants. Because of its sensitivity and quantitative nature, iPool-Seq can be applied to any insertional mutagenesis library and is especially suitable for genetically complex setups like pooled infections of eukaryotic hosts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.