ArticleRNA biology2018
Primary transcriptome analysis reveals importance of IS elements for the shaping of the transcriptional landscape of Bordetella pertussis.
Article in RNA biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Exploring the IS-capades ofMicrobial genomics · 2026Article
- An IS element-driven antisense RNA attenuates the expression of serotype 2 fimbriae and the cytotoxicity ofEmerging microbes & infections · 2025Article
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- Global analysis of the Hfq-mediated RNA interactome discovers a MicA homolog that affects the cytotoxicity, biofilm formation, and resistance to complement of Bordetella pertussis.Nucleic acids research · 2025Article
- A role for genomics-based studies of Bordetella pertussis adaptation.Current opinion in infectious diseases · 2025Review
- Combined transcriptomic and ChIPseq analyses of themSystems · 2024Article
- Investigating the impact of insertion sequences and transposons in the genomes of the most significant phytopathogenic bacteria.Microbial genomics · 2024Article
- Avirulent phenotype promotesEmerging microbes & infections · 2023Article
- Interference of flagellar rotation up-regulates the expression of small RNA contributing toScience advances · 2022Article
- Insertion Sequence (IS)-Excision Enhancer (IEE)-Mediated IS Excision from theApplied and environmental microbiology · 2022Article
- Towards comprehensive understanding of bacterial genetic diversity: large-scale amplifications inMicrobial genomics · 2022Article
- Maturation of 23S rRNA includes removal of helix H1 in many bacteria.RNA biology · 2021Article
- Identification of BvgA-Dependent and BvgA-Independent Small RNAs (sRNAs) in Bordetella pertussis Using the Prokaryotic sRNA Prediction Toolkit ANNOgesic.Microbiology spectrum · 2021Article
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- Article
- Radical genome remodelling accompanied the emergence of a novel host-restricted bacterial pathogen.PLoS pathogens · 2021Article
- Omics Analysis of Blood-Responsive Regulon inInternational journal of molecular sciences · 2021Article
- Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution.Emerging infectious diseases · 2021Article
- A Mutation Upstream of themSystems · 2020Article
- Impact of insertion sequences on convergent evolution of Shigella species.PLoS genetics · 2020Article
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bordetella pertussis is the causative agent of whooping cough, a respiratory disease still considered as a major public health threat and for which recent re-emergence has been observed. Constant reshuffling of Bordetella pertussis genome organization was observed during evolution. These rearrangements are essentially mediated by Insertion Sequences (IS), a mobile genetic elements present in more than 230 copies in the genome, which are supposed to be one of the driving forces enabling the pathogen to escape from vaccine-induced immunity. Here we use high-throughput sequencing approaches (RNA-seq and differential RNA-seq), to decipher Bordetella pertussis transcriptome characteristics and to evaluate the impact of IS elements on transcriptome architecture. Transcriptional organization was determined by identification of transcription start sites and revealed also a large variety of non-coding RNAs including sRNAs, leaderless mRNAs or long 3' and 5'UTR including seven riboswitches. Unusual topological organizations, such as overlapping 5'- or 3'-extremities between oppositely orientated mRNA were also unveiled. The pivotal role of IS elements in the transcriptome architecture and their effect on the transcription of neighboring genes was examined. This effect is mediated by the introduction of IS harbored promoters or by emergence of hybrid promoters. This study revealed that in addition to their impact on genome rearrangements, most of the IS also impact on the expression of their flanking genes. Furthermore, the transcripts produced by IS are strain-specific due to the strain to strain variation in IS copy number and genomic context.
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