ArticleFuture science OA2018
Validation of quantitative polymerase chain reaction with Southern blot method for telomere length analysis.
Article in Future science OA, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Do the telomere ends justify the physical means?Journal of the American Geriatrics Society · 2021Article
- Subclinical atherosclerosis and immune activation in young HIV-infected patients with telomere shortening.Aging · 2021Article
- Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.PloS one · 2021Article
- Telomere Attrition in Neurodegenerative Disorders.Frontiers in cellular neuroscience · 2020Article
- Association of birth outcomes and postnatal growth with adult leukocyte telomere length: Data from New Delhi Birth Cohort.Maternal & child nutrition · 2019Article
- Hazardous air pollutants and telomere length in the Sister Study.Environmental epidemiology (Philadelphia, Pa.) · 2019Article
- Article
- CD4+ memory T cells infected with latent HIV-1 are susceptible to drugs targeting telomeres.Cell cycle (Georgetown, Tex.) · 2018Article
- The Alteration of Subtelomeric DNA Methylation in Aging-Related Diseases.Frontiers in genetics · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTelomere length (TL) measurement by quantitative polymerase chain reaction (PCR) has been widely accepted, but limited information regarding its validation with a gold-standard technique is available. MATERIALS &
methodsWe measured TL by Southern blot and monochrome multiplex quantitative PCR (MMqPCR) and validated the results of TL in leukocytes of 94 participants with mean age 43.2 years, BMI 19-41 (mean 27.8 ± 4.3) kg/m
resultsA significant positive correlation was observed between TL measured by MMqPCR and Southern blot assay (correlation coefficient r = +0.896, p < 0.0001). The inter- and intra-assay CVs of the MMqPCR assay were 5.3 and 4.07%, respectively.
conclusionWe observed that experimental discrepancies have an impact on TL analysis and there is a need to improve the optimum conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.