ArticleStructure (London, England : 1993)2018
Correlative Microscopy of Vitreous Sections Provides Insights into BAR-Domain Organization In Situ.
Article in Structure (London, England : 1993), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Article
- Recovery of plasma membrane tension after a hyperosmotic shock.Molecular biology of the cell · 2025Article
- Structural biology inside multicellular specimens using electron cryotomography.Quarterly reviews of biophysics · 2025Review
- Definition of phosphatidylinositol 4,5-bisphosphate distribution by freeze-fracture replica labeling.The Journal of cell biology · 2025Article
- Article
- Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix.The Journal of cell biology · 2024Article
- Article
- High-resolution structure determination using high-throughput electron cryo-tomography.Acta crystallographica. Section D, Structural biology · 2022Review
- Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.Annual review of biochemistry · 2022Review
- Article
- CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy.Journal of structural biology · 2021Article
- Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography.Nature communications · 2021Article
- Preservation of Fluorescence Signal and Imaging Optimization for Integrated Light and Electron Microscopy.Frontiers in cell and developmental biology · 2021Article
- Plasma Membrane MCC/Eisosome Domains Promote Stress Resistance in Fungi.Microbiology and molecular biology reviews : MMBR · 2020Review
- Article
- Regulation of nutrient transporters by metabolic and environmental stresses.Current opinion in cell biology · 2020Review
- Reliable estimation of membrane curvature for cryo-electron tomography.PLoS computational biology · 2020Article
- Mechanisms of negative membrane curvature sensing and generation by ESCRT III subunit Snf7.Protein science : a publication of the Protein Society · 2020Article
- Plasma membrane tension regulates eisosome structure and function.Molecular biology of the cell · 2020Article
- Investigating eukaryotic cells with cryo-ET.Molecular biology of the cell · 2020Article
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3 authors.
Funding
Abstract
Electron microscopy imaging of macromolecular complexes in their native cellular context is limited by the inherent difficulty to acquire high-resolution tomographic data from thick cells and to specifically identify elusive structures within crowded cellular environments. Here, we combined cryo-fluorescence microscopy with electron cryo-tomography of vitreous sections into a coherent correlative microscopy workflow, ideal for detection and structural analysis of elusive protein assemblies in situ. We used this workflow to address an open question on BAR-domain coating of yeast plasma membrane compartments known as eisosomes. BAR domains can sense or induce membrane curvature, and form scaffold-like membrane coats in vitro. Our results demonstrate that in cells, the BAR protein Pil1 localizes to eisosomes of varying membrane curvature. Sub-tomogram analysis revealed a dense protein coat on curved eisosomes, which was not present on shallow eisosomes, indicating that while BAR domains can assemble at shallow membranes in vivo, scaffold formation is tightly coupled to curvature generation.
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