ArticleCell systems2018
Computational Re-design of Synthetic Genetic Oscillators for Independent Amplitude and Frequency Modulation.
Article in Cell systems, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Self-Oscillatory Neuron-like Devices for Unconventional Computing Applications.Chemical reviews · 2026Review
- Expanding the toolkit of LacI/GalR chimeras.PloS one · 2026Article
- Intracellularly coupled oscillators for synthetic biology.Nature communications · 2025Review
- A tool for modeling gene regulatory networks (GRN_modeler) and its applications to synthetic biology.Molecular systems biology · 2025Article
- The dynamic-process characterization and prediction of synthetic gene circuits by dynamic delay model.iScience · 2024Article
- Bacterial degrons in synthetic circuits.Open biology · 2022Review
- A protocol for dynamic model calibration.Briefings in bioinformatics · 2022Article
- Independent control of amplitude and period in a synthetic oscillator circuit with modified repressilator.Communications biology · 2022Article
- A frequency-amplitude coordinator and its optimal energy consumption for biological oscillators.Nature communications · 2021Article
- Predictive biology: modelling, understanding and harnessing microbial complexity.Nature reviews. Microbiology · 2020Review
- Application of combinatorial optimization strategies in synthetic biology.Nature communications · 2020Review
- Computer-Aided Whole-Cell Design: Taking a Holistic Approach by Integrating Synthetic With Systems Biology.Frontiers in bioengineering and biotechnology · 2020Article
- Bacterial variability in the mammalian gut captured by a single-cell synthetic oscillator.Nature communications · 2019Article
- Massive computational acceleration by using neural networks to emulate mechanism-based biological models.Nature communications · 2019Article
- Synthetic Switches and Regulatory Circuits in Plants.Plant physiology · 2019Review
- Computational analysis of viable parameter regions in models of synthetic biological systems.Journal of biological engineering · 2019Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
To perform well in biotechnology applications, synthetic genetic oscillators must be engineered to allow independent modulation of amplitude and period. This need is currently unmet. Here, we demonstrate computationally how two classic genetic oscillators, the dual-feedback oscillator and the repressilator, can be re-designed to provide independent control of amplitude and period and improve tunability-that is, a broad dynamic range of periods and amplitudes accessible through the input "dials." Our approach decouples frequency and amplitude modulation by incorporating an orthogonal "sink module" where the key molecular species are channeled for enzymatic degradation. This sink module maintains fast oscillation cycles while alleviating the translational coupling between the oscillator's transcription factors and output. We characterize the behavior of our re-designed oscillators over a broad range of physiologically reasonable parameters, explain why this facilitates broader function and control, and provide general design principles for building synthetic genetic oscillators that are more precisely controllable.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.