Evidence map›Paper›PMID 29679176›Full record

ArticleRice (New York, N.Y.)2018

A Versatile Vector Toolkit for Functional Analysis of Rice Genes.

Feng He, Fan Zhang, Wenxian Sun, Yuese Ning, Guo-Liang Wang

Erratum issuedAbstract read
In one paragraph

Article in Rice (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 66 papers.

0numbers the graph read from it
0cells of the map it votes in
66citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

66 citing papers in PubMed.

  1. Article
  2. OsCPK12-OsATPD1 Module Regulates Photosynthetic Acclimation to Light Stress in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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  5. OsRNase ZPlant communications · 2026
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  19. Plants (Basel, Switzerland) · 2025
    Article
  20. Article

6 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Feng HeState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Fan ZhangState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Wenxian SunCollege of Plant Protection, China Agricultural University, Beijing, 100193, China.
Yuese NingState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. ningyuese@caas.cn.
Guo-Liang WangState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. wang.620@osu.edu.ORCID http://orcid.org/0000-0001-8877-3064

Funding

National Key Research and Development Program of China 2016YFD0100600National Natural Science Foundation of China 31571944Young Elite Scientist Sponsorship of China Association for Science and Technology 2015QNRC001
6 · The paper itself

Abstract

backgroundRice (Oryza sativa) is the main food for half of the world's population, and is considered the model for molecular biology studies of monocotyledon species. Although the rice genome was completely sequenced about 15 years ago, the function of most rice genes is still unknown.

resultsIn this study, we developed a vector toolkit that contains 42 vectors for transient expression studies in rice protoplasts and stable expression analysis in transgenic rice. These vectors have been successfully used to study protein subcellular localization, protein-protein interaction, gene overexpression, and the CRISPR/Cas9-mediated gene editing. A novel feature of these vectors is that they contain a universal multiple cloning site, which enables more than 99% of the rice coding sequences to be conveniently transferred between vectors.

conclusionsThe versatile vectors represent a highly efficient and high-throughput toolkit for functional analysis of rice genes.

Indexed as

Binary vectorCRISPR/Cas9Oryza sativaTransient expressionVector toolkit

Identifiers

PMID29679176
PMCPMC5910328

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.