ArticleNature communications2018
Self-replication of DNA by its encoded proteins in liposome-based synthetic cells.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
117 citing papers in PubMed, 258 citations in OpenAlex.
- Combinatorial Optimization of Protein Systems in Synthetic Cells.ACS synthetic biology · 2026Article
- Uniform Microscale Thermoresponsive Liposomes for Controlled Release Above Body Temperature.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A self-replicating artificial module-genome that generates bacterial chromosome replication system in vitro.Nucleic acids research · 2026Article
- Image-based phenotypic sorting of synthetic cells.Science advances · 2026Article
- Autocatalytic selection of gene functions in synthetic cells.Communications biology · 2026Article
- PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized proteins.Nature communications · 2026Article
- Targeting Neurovascular Pathways: Gene Therapy as a Novel Treatment for Vascular Dementia After Stroke.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2026Review
- Bottom-up Strategies for Generating Polymer Protocells That Mimic Cellular Communication.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- A synthetic cell with integrated DNA self-replication and lipid biosynthesis.Nature communications · 2026Article
- Lipid composition effects on the number and size of liposomes formed by the inverted emulsion method.Biophysical journal · 2026Article
- Composition dependent effects of charged lipids on freeze-thaw induced fusion in dioleoyl phospholipid membranes.Biophysics and physicobiology · 2026Article
- Autonomous biogenesis of all thirty proteins of the Escherichia coli translation machinery.Nature communications · 2025Article
- Reconstituting transcription-translation-coupled DNA replication within complex in vitro biological systems.Nature communications · 2025Article
- Orchestrating Self-Replication in Artificial Cells with Digital Microfluidics.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Synthetic cells by the numbers.iScience · 2025Review
- Genetically Encoded Control ofACS synthetic biology · 2025Article
- Strategies and applications of synthetic cell communication.Nature chemical biology · 2025Review
- Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method.Nature communications · 2025Article
- Dynamic Transcription Machineries in Protocells.Journal of the American Chemical Society · 2025Review
- Scaling Up Synthetic Cell Production Using Robotics and Machine Learning Toward Therapeutic Applications.Advanced biology · 2025Article
57 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replication of DNA-encoded information and its conversion into functional proteins are universal properties of life. In an effort toward the construction of a synthetic minimal cell, we implement here the DNA replication machinery of the Φ29 virus in a cell-free gene expression system. Amplification of a linear DNA template by self-encoded, de novo synthesized Φ29 proteins is demonstrated. Complete information transfer is confirmed as the copied DNA can serve as a functional template for gene expression, which can be seen as an autocatalytic DNA replication cycle. These results show how the central dogma of molecular biology can be reconstituted and form a cycle in vitro. Finally, coupled DNA replication and gene expression is compartmentalized inside phospholipid vesicles providing the chassis for evolving functions in a prospective synthetic cell relying on the extant biology.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.