ArticleToxicology in vitro : an international journal published in association with BIBRA2018
Fetal bovine serum induces sustained, but reversible, epithelial-mesenchymal transition in the BEAS-2B cell line.
Article in Toxicology in vitro : an international journal published in association with BIBRA, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
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- Polyinosinic:polycytidylic acid causes epithelial-mesenchymal transition via BAFF expression in Beas-2B human bronchial epithelial cells.International journal of medical sciences · 2026Article
- Advancing Environmental ToxicologyEnvironment & health (Washington, D.C.) · 2024Review
- Establishment and comparison of human term placenta-derived trophoblast cells†.Biology of reproduction · 2024Article
- Aerobic glycolysis of bronchial epithelial cells rewiresInfection and immunity · 2024Article
- Ezrin Contributes to the Damage of Airway Epithelial Barrier Related to Diabetes Mellitus.Journal of inflammation research · 2024Article
- Arsenic and Human Health: New Molecular Mechanisms For Arsenic-Induced Cancers.Current pollution reports · 2023Article
- miRNAs and arsenic-induced carcinogenesis.Advances in pharmacology (San Diego, Calif.) · 2023Review
- 3-Nitrobenzanthrone promotes malignant transformation in human lung epithelial cells through the epiregulin-signaling pathway.Cell biology and toxicology · 2022Article
- Toxicity of Water- and Organic-Soluble Wood Tar Fractions from Biomass Burning in Lung Epithelial Cells.Chemical research in toxicology · 2021Article
- The differential effects of commercial specialized media on cell growth and transforming growth factor beta 1-induced epithelial-mesenchymal transition in bronchial epithelial cells.Molecular biology reports · 2020Article
- Isoprene-Derived Secondary Organic Aerosol Induces the Expression of MicroRNAs Associated with Inflammatory/Oxidative Stress Response in Lung Cells.Chemical research in toxicology · 2020Article
- Hypoxia-induced cancer stemness acquisition is associated with CXCR4 activation by its aberrant promoter demethylation.BMC cancer · 2019Article
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3 authors.
Funding
Abstract
BEAS-2B is a non-malignant, immortalized human cell line that has been used extensively as a model of lung epithelium. Despite ATCC recommendations to culture BEAS-2B in defined, serum-free media, many publications describe culturing BEAS-2B in fetal bovine serum (FBS)-containing media. The objective of this study was to define the effects of FBS on BEAS-2B cells. FBS exposure resulted in increased nuclear levels of transcription factors responsible for regulating epithelial-mesenchymal transition (EMT), increased cell invasiveness and increased anchorage-independent growth. FBS-exposed BEAS-2B cells exhibited a decrease of the epithelial markers, E-cadherin and claudin-1 at the mRNA and protein levels, along with a corresponding increase of the mesenchymal marker, vimentin, at the protein level. Fractionation studies implicated an active moiety in FBS with a molecular weight larger than 30 kD. The mesenchymal phenotype was persistent provided FBS exposure was maintained. Upon FBS removal, both epithelial and mesenchymal markers began to revert toward an epithelial phenotype. Transforming growth factor β1 (TGFβ1) exposure to BEAS-2B recapitulated some key features of FBS-induced EMT. Our data suggest that FBS-exposed BEAS-2B cells do not accurately model the epithelial phenotype. Interpretation of data from BEAS-2B should include careful consideration of the effect of culture conditions.
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