Evidence map›Paper›PMID 29677652›Full record

ArticleCardiovascular pathology : the official journal of the Society for Cardiovascular Pathology

Cardiomyocytes have mosaic patterns of protein expression.

Tony Y Wang, Dongwon Lee, Karen Fox-Talbot, Dan E Arking, Aravinda Chakravarti, Marc K Halushka

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.

  1. Pooled it
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  8. An expanded proteome of cardiac t-tubules.Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Tony Y WangDepartment of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Dongwon LeeMcKusick - Nathans Institute of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Karen Fox-TalbotDepartment of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Dan E ArkingMcKusick - Nathans Institute of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Aravinda ChakravartiMcKusick - Nathans Institute of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Marc K HalushkaDepartment of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA. Electronic address: mhalush1@jhmi.edu.
Johns Hopkins University · USJohns Hopkins Medicine · US

Funding

Genome-Wide Association Analysis in Essential Hypertension (FEHGAS study)R01HL086694 · NHLBI · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI ARAVINDA CHAKRAVARTI · 2007 to 2026
$21.2M
Functional Dissection of the Sudden Cardiac Death Associated BAZ2B locusR01HL111267 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI ARKING, DAN E · 2012 to 2016
$3.6M
Fine Dissection of Atherosclerosis Microenvironment RNA ExpressionR01HL137811 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI HALUSHKA, MARC K · 2017 to 2020
$2.4M
NHLBI NIH HHS R01 HL086694NHLBI NIH HHS R01 HL111267NHLBI NIH HHS R01 HL137811
6 · The paper itself

Abstract

Skeletal myocytes have well-established fast and slow twitch fibers with unique gene and protein specific expression patterns. By immunohistochemical staining, these show a mosaic pattern across myocytes. We hypothesized cardiac myocytes may behave similarly where some proteins are differentially expressed between mature cardiomyocytes. We utilized the tool HPASubC on over 52,000 cardiac images of the Human Protein Atlas to identify differential protein expression patterns by immunohistochemistry across the cardiomyocytes. We matched identified proteins to open chromatin and gene expression data. We identified 143 putative proteins with mosaic patterns of expression across the cardiomyocytes. We validated four of these proteins (MYL3, MYL4, PAM, and MYOM1) and demonstrated unique atrial or ventricular patterns of expression for each. Acetylation of histone H3K27 at the promoters of these four genes were consistent with the atrial/ventricular expression patterns. Despite the generally accepted homogeneity of cardiomyocytes, a small subset of proteins varies between cardiomyocytes in a mosaic pattern. This fundamental process has been previously uncharacterized. These changes may inform on different functional and disease-related activities of proteins in individual cardiomyocytes.

Indexed as

AcetylationAmidine-LyasesConnectinGene Expression RegulationHistonesHumansImage Processing, Computer-AssistedImmunohistochemistryMixed Function OxygenasesMosaicismMuscle ProteinsMyocytes, CardiacMyosin Light ChainsPattern Recognition, AutomatedPhenotypePromoter Regions, GeneticAmidine-LyasesConnectinHistonesMixed Function OxygenasesMuscle ProteinsMYL4 protein, humanMYOM1 protein, humanmyosin light chain 3, humanMyosin Light ChainsPAM protein, humanCardiomyocyteExpressionMyomesinMyosin light chainProteomics

Identifiers

PMID29677652
PMCPMC5940500
OpenAlexW2793813722

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.