ArticleBiomaterials2018
Investigating the interplay between substrate stiffness and ligand chemistry in directing mesenchymal stem cell differentiation within 3D macro-porous substrates.
Article in Biomaterials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Ribbon-shaped microgels as bioinks for 3D bioprinting of anisotropic tissue structures.Bioactive materials · 2026Article
- Mechanobiological Dynamics-Inspired Mechanomodulatory Biomaterials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Donor Variability and 3D Culture Models Influence Human Mesenchymal Stem Cell Differentiation.Tissue engineering. Part A · 2026Article
- The Rise of Mechanobiology for Advanced Cell Engineering and Manufacturing.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Engineered Protein Hydrogels as Biomimetic Cellular Scaffolds.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Structurally and mechanically tuned macroporous hydrogels for scalable mesenchymal stem cell-extracellular matrix spheroid production.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Role of durotomy on function outcome, tissue sparing, inflammation, and tissue stiffness after spinal cord injury in rats.MedComm · 2024Article
- Identification of potential biomarkers for aging diagnosis of mesenchymal stem cells derived from the aged donors.Stem cell research & therapy · 2024Article
- Segmenting mechanically heterogeneous domains via unsupervised learning.Biomechanics and modeling in mechanobiology · 2024Article
- The construction of elastin-like polypeptides and their applications in drug delivery system and tissue repair.Journal of nanobiotechnology · 2023Review
- Fabrication of lumen-forming colorectal cancer organoids using a newly designed laminin-derived bioink.International journal of bioprinting · 2023Article
- Integrating physicomechanical and biological strategies for BTE: biomaterials-induced osteogenic differentiation of MSCs.Theranostics · 2023Review
- Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures.Engineering in life sciences · 2022Article
- Decellularized Human Umbilical Tissue-Derived Hydrogels Promote Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells.Bioengineering (Basel, Switzerland) · 2022Article
- Relationship of matrix stiffness and cell morphology in regulation of osteogenesis and adipogenesis of BMSCs.Molecular biology reports · 2022Article
- Pliable, Scalable, and Degradable Scaffolds with Varying Spatial Stiffness and Tunable Compressive Modulus Produced by Adopting a Modular Design Strategy at the Macrolevel.ACS polymers Au · 2021Article
- Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture.ACS biomaterials science & engineering · 2021Review
- Protein Hydrogels: The Swiss Army Knife for Enhanced Mechanical and Bioactive Properties of Biomaterials.Nanomaterials (Basel, Switzerland) · 2021Review
- Cell encapsulated and microenvironment modulating microbeads containing alginate hydrogel system for bone tissue engineering.Progress in biomaterials · 2021Article
- Porous polyetheretherketone microcarriers fabricated via hydroxylation together with cell-derived mineralized extracellular matrix coatings promote cell expansion and bone regeneration.Regenerative biomaterials · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Dimensionality can have a profound impact on stiffness-mediated differentiation of mesenchymal stem cells (MSCs). However, while we have begun to understand cellular response when encapsulated within 3D substrates, the behavior of cells within macro-porous substrates is relatively underexplored. The goal of this study was to determine the influence of macro-porous topographies on stiffness-mediated differentiation of MSCs. We developed macro-porous recombinant elastin-like protein (ELP) substrates that allow independent control of mechanical properties and ligand chemistry. We then used computational modeling to probe the impact of pore topography on the mechanical stimulus that cells are exposed to within these substrates, and finally we investigated stiffness induced biases towards adipogenic and osteogenic differentiation of MSCs within macro-porous substrates. Computational modeling revealed that there is significant heterogeneity in the mechanical stimuli that cells are exposed to within porous substrates and that this heterogeneity is predominantly due to the wide range of possible cellular orientations within the pores. Surprisingly, MSCs grown within 3D porous substrates respond to increasing substrate stiffness by up-regulating both osteogenesis and adipogenesis. These results demonstrate that within porous substrates the behavior of MSCs diverges from previously observed responses to substrate stiffness, emphasizing the importance of topography as a determinant of cellular behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.