Evidence map›Paper›PMID 29677003›Full record

ArticleCornea2018

Identification of a Novel TCF4 Isoform in the Human Corneal Endothelium.

Allen O Eghrari, Shivakumar Vasanth, Briana C Gapsis, Henry Bison, Ula Jurkunas, S Amer Riazuddin, John D Gottsch

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cornea, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Allen O EghrariThe Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD.
Shivakumar VasanthThe Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD.
Briana C GapsisThe Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD.
Henry BisonThe Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD.
Ula JurkunasSchepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston MA.
S Amer RiazuddinThe Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD.
John D GottschThe Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD.
Johns Hopkins University · USJohns Hopkins Medicine · US

Funding

Wilmer Inst. Mentored Clinical Research Scholar ProgramK12EY015025 · NEI · JOHNS HOPKINS UNIVERSITY · PI James T Handa, Harry Alan Quigley · 2003 to 2026
$8.6M
Genetics of Fuchs Corneal DystrophyR01EY016835 · NEI · JOHNS HOPKINS UNIVERSITY · PI GOTTSCH, JOHN D · 2007 to 2017
$5.7M
NEI NIH HHS K12 EY015025NEI NIH HHS R01 EY016835
6 · The paper itself

Abstract

purposeAlternative splice isoforms of TCF4, a gene implicated in Fuchs corneal dystrophy, have been identified in multiple human tissues outside of the eye. The aim of this study was to identify the transcriptional profile of TCF4 in the corneal endothelium.

methodsWe extracted RNA from the donor corneal endothelium and performed rapid amplification of cDNA ends. We tested the expression pattern of 1 newly identified isoform (7b) in a panel of cDNA derived from multiple human tissues and included cDNA from corneal endothelial (CE) and retinal pigment epithelial cell lines. To further delineate differential expression of TCF4 splice variants that span CTG18.1, we analyzed expression of 6 alternative splice isoforms that are transcribed from either exon 2 or 3 in RNA extracted from the corneal endothelium of 3 normal donors and a CE cell line.

resultsWe identified 11 different isoforms in control CE tissue, including 1 isoform (7b) not reported previously. This isoform is enriched specifically in the corneal endothelium and placenta compared with other tissues in a panel of human cDNA.

conclusionsWe demonstrate the complex expression profile of TCF4 in the human corneal endothelium and reveal expression of alternative splice variants of TCF4.

Indexed as

AgedDNA, ComplementaryEndothelium, CornealFemaleFuchs' Endothelial DystrophyGene Expression ProfilingGenetic Predisposition to DiseaseGenotypeHumansMaleMiddle AgedProtein IsoformsTranscription Factor 4Trinucleotide Repeat ExpansionDNA, ComplementaryProtein IsoformsTCF4 protein, humanTranscription Factor 4

Identifiers

PMID29677003
PMCPMC6482941
OpenAlexW2800657709

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.