ArticleChembiochem : a European journal of chemical biology2018
Specific Spatial Localization of Actin and DNA in a Water/Water Microdroplet: Self-Emergence of a Cell-Like Structure.
Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- Molecular Crowding Stabilizes DNA Coacervate Droplets and Generates Self-Organized Characteristic Patterns.JACS Au · 2026Article
- Relationship Between Filament-Polymerizing Muscle Myosin and Droplets Generated by Liquid-Liquid Phase Separation.Chembiochem : a European journal of chemical biology · 2026Article
- A Facile Platform for One-Step Generation of Uniform Microdroplets through Dehydration-Driven Phase Separation in Microfluidics.Small methods · 2026Article
- Spontaneous Fusion of Aqueous Two-Phase Droplets: Effect of Coexisting Phospholipids on Stability.Chembiochem : a European journal of chemical biology · 2025Article
- Article
- Metastable phase-separated droplet generation and long-time DNA enrichment by laser-induced Soret effect.Communications chemistry · 2025Article
- Self-growing protocell models in aqueous two-phase system induced by internal DNA replication reaction.Nature communications · 2025Article
- Accelerated amyloid fibril formation at the interface of liquid-liquid phase-separated droplets by depletion interactions.Protein science : a publication of the Protein Society · 2025Article
- Artificial cells with all-aqueous droplet-in-droplet structures for spatially separated transcription and translation.Nature communications · 2025Article
- Single-molecule characterization of target search dynamics of DNA-binding proteins in DNA-condensed droplets.Nucleic acids research · 2023Article
- Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation.Communications chemistry · 2023Article
- Biomolecular Liquid-Liquid Phase Separation for Biotechnology.Biotech (Basel (Switzerland)) · 2023Review
- The Role of Liquid-Liquid Phase Separation in Actin Polymerization.International journal of molecular sciences · 2023Review
- Article
- Water-in-Water Droplets Selectively Uptake Self-Assembled DNA Nano/Microstructures: a Versatile Method for Purification in DNA Nanotechnology.Chembiochem : a European journal of chemical biology · 2022Article
- Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation.Nature communications · 2022Article
- Progress on Crowding Effect in Cell-like Structures.Membranes · 2022Review
- Emergence of uniform linearly-arranged micro-droplets entrapping DNA and living cells through water/water phase-separation.Scientific reports · 2021Article
- Physics of compartmentalization: How phase separation and signaling shape membrane and organelle identity.Computational and structural biotechnology journal · 2021Review
- Higher-order structure of DNA determines its positioning in cell-size droplets under crowded conditions.PloS one · 2021Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of binary hydrophilic polymers on a pair of representative bio-macromolecules in a living cell has been examined. The results showed that these bio-macromolecules exhibited specific localization in cell-sized droplets that were spontaneously formed through water/water microphase segregation under crowding conditions with coexisting polymers. In these experiments, a simple binary polymer system with poly(ethylene glycol) (PEG) and dextran (DEX) was used. Under the conditions of microphase segregation, DNA was entrapped within cell-sized droplets rich in DEX. Similarly, F-actin, linearly polymerized actin, was entrapped specifically within microdroplets rich in DEX, whereas G-actin, a monomeric actin, was distributed evenly inside and outside these droplets. This study has been extended to a system with both F-actin and DNA, and it was found that DNA molecules were localized separately from aligned F-actin proteins to create microdomains inside microdroplets, reflecting the self-emergence of a cellular morphology similar to a stage of cell division.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.