ArticleArchives of virology2018
A multiplex PCR method for the simultaneous detection of three viruses associated with canine viral enteric infections.
Article in Archives of virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Multiple etiological agents associated with the deaths of trafficked pangolins.Zoological research · 2026Article
- Automated highly multiplex detection system for respiratory pathogens in canines.Frontiers in veterinary science · 2025Article
- Molecular detection of canine viral infectious diseases in China: an investigation from 2018 to 2024.Frontiers in veterinary science · 2025Article
- Molecular detection and quantification of canine parvovirus 2 using a fast and sensitive SYBRVeterinary world · 2024Article
- Multiplex One-Step RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Enteric Viruses of Dogs and Cats.Viruses · 2023Article
- Fatal canine parvovirus type 2a and 2c infections in wild Chinese pangolins (Manis pentadactyla) in southern China.Transboundary and emerging diseases · 2022Article
- Canine Distemper Virus in Autochtonous and Imported Dogs, Southern Italy (2014-2021).Animals : an open access journal from MDPI · 2022Article
- Development of Multiplex PCR Assay for Screening of T6SS-5 Gene Cluster: TheDiagnostics (Basel, Switzerland) · 2022Article
- Canine circovirus and Canine adenovirus type 1 and 2 in dogs with parvoviral enteritis.Veterinary research communications · 2022Article
- Molecular characterization of Cachavirus firstly detected in dogs in China.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2020Article
- One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.Molecular and cellular probes · 2020Article
- An Improved Polymerase Cross-Linking Spiral Reaction Assay for Rapid Diagnostic of Canine Parvovirus 2 Infection.Frontiers in veterinary science · 2020Article
- Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus.Archives of virology · 2019Article
- Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2.BMC veterinary research · 2019Article
- Multiplex PCR methods for detection of several viruses associated with canine respiratory and enteric diseases.PloS one · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
The aim of this study was to establish a multiplex PCR (mPCR) method that can simultaneously detect canine parvovirus (CPV-2), canine coronavirus (CCoV) and canine adenovirus (CAV), thereby eliminating the need to detect these pathogens individually. Based on conserved regions in the genomes of these three viruses, the VP2 gene of CPV-2, the endoribonuclease nsp15 gene of CCoV, and the 52K gene of CAV were selected for primer design. The specificity of the mPCR results showed no amplification of canine distemper virus (CDV), canine parainfluenza virus (CPIV), or pseudorabies virus (PRV), indicating that the method had good specificity. A sensitivity test showed that the detection limit of the mPCR method was 1 × 10
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