ArticleEuropean biophysics journal : EBJ2018
Multi-wavelength analytical ultracentrifugation of human serum albumin complexed with porphyrin.
Article in European biophysics journal : EBJ, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Organic-solvent mediated self-assembly of protoporphyrin IX with human serum albumin.International journal of biological macromolecules · 2025Article
- Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments.Current protocols · 2024Article
- AAV analysis by sedimentation velocity analytical ultracentrifugation: beyond empty and full capsids.European biophysics journal : EBJ · 2023Article
- Multi-wavelength analytical ultracentrifugation of biopolymer mixtures and interactions.Analytical biochemistry · 2022Review
- Multi-wavelength analytical ultracentrifugation as a tool to characterise protein-DNA interactions in solution.European biophysics journal : EBJ · 2020Article
- A calibration disk for the correction of radial errors from chromatic aberration and rotor stretch in the Optima AUC™ analytical ultracentrifuge.European biophysics journal : EBJ · 2020Article
- Analytical ultracentrifuge: an ideal tool for characterization of non-coding RNAs.European biophysics journal : EBJ · 2020Review
- Moving analytical ultracentrifugation software to a good manufacturing practices (GMP) environment.PLoS computational biology · 2020Article
- In Vitro Analytical Approaches to Study Plant Ligand-Receptor Interactions.Plant physiology · 2020Review
- Probing RNA-Protein Interactions and RNA Compaction by Sedimentation Velocity Analytical Ultracentrifugation.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Novel combinations of experimental and computational analysis tested on the binding of metalloprotoporphyrins to albumin.International journal of biological macromolecules · 2019Article
- Measuring molecular interactions in solution using multi-wavelength analytical ultracentrifugation: combining spectral analysis with hydrodynamics.The biochemist · 2019Article
- Analytical ultracentrifugation (AUC): a seminal tool offering multiple solutions.European biophysics journal : EBJ · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The new Beckman Coulter Optima AUC instrument, which features multi-wavelength detection that couples the hydrodynamic separation of colloidal mixtures to spectral deconvolution of interacting and non-interacting solutes present in a mixture, was used to analyze the composition of human serum albumin (HSA) bound to metallo-protoporphyrin. We present new methods implemented in UltraScan that permit Optima AUC-derived multi-wavelength data to be spectrally decomposed in the same fashion as has been made possible for the Cölfen detector earlier. We demonstrate this approach by spectrally separating sedimentation velocity experimental data from mixtures of apo-HSA and HSA complexed to different metallo-protoporphyrins. We further demonstrate how multi-wavelength AUC can accurately recover percentages of metallo-protoporphyrin-bound HSA and apo-HSA from mixtures and how multi-wavelength AUC permits the calculation of molar extinction coefficients for porphyrins bound to HSA. The presented method has broad applicability to other complex systems where mixtures of molecules with different spectral properties need to be characterized.
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Registered trials
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