ArticleScience advances2018
Low-input and multiplexed microfluidic assay reveals epigenomic variation across cerebellum and prefrontal cortex.
Article in Science advances, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- Sex-specific role of the 5-HTNature communications · 2025Article
- Lossless Altered Histone Modification Analysis System (LAHMAS).Lab on a chip · 2025Article
- scNanoSeq-CUT&Tag: a single-cell long-read CUT&Tag sequencing method for efficient chromatin modification profiling within individual cells.Nature methods · 2024Article
- Single-Cell RNA Sequencing and Combinatorial Approaches for Understanding Heart Biology and Disease.Biology · 2024Review
- Article
- Epigenomic tomography for probing spatially defined chromatin state in the brain.Cell reports methods · 2024Article
- nMOWChIP-seq: low-input genome-wide mapping of non-histone targets.NAR genomics and bioinformatics · 2022Article
- Cell-type-specific epigenomic variations associated withNAR genomics and bioinformatics · 2022Article
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- Prolonged epigenomic and synaptic plasticity alterations following single exposure to a psychedelic in mice.Cell reports · 2021Article
- Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division.Nature communications · 2021Article
- Profiling Chromatin Accessibility at Single-cell Resolution.Genomics, proteomics & bioinformatics · 2021Review
- Microfabrication with Very Low-Average Power of Green Light to Produce PDMS Microchips.Polymers · 2021Article
- Mutational profiling of micro-dissected pre-malignant lesions from archived specimens.BMC medical genomics · 2020Article
- Multiplexed and Ultralow-Input ChIP-seq Enabled by Tagmentation-Based Indexing and Facile Microfluidics.Analytical chemistry · 2020Article
- Large eQTL meta-analysis reveals differing patterns between cerebral cortical and cerebellar brain regions.Scientific data · 2020Article
- Chromatin integration labeling for mapping DNA-binding proteins and modifications with low input.Nature protocols · 2020Article
- Scalable Signature-Based Molecular Diagnostics Through On-chip Biomarker Profiling Coupled with Machine Learning.Annals of biomedical engineering · 2020Review
- Translational Opportunities for Microfluidic Technologies to Enable Precision Epigenomics.Analytical chemistry · 2020Article
- A parallelized, automated platform enabling individual or sequential ChIP of histone marks and transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Extensive effort is under way to survey the epigenomic landscape of primary ex vivo tissues to establish normal reference data and to discern variation associated with disease. The low abundance of some tissue types and the isolation procedure required to generate a homogenous cell population often yield a small quantity of cells for examination. This difficulty is further compounded by the need to profile a myriad of epigenetic marks. Thus, technologies that permit both ultralow input and high throughput are desired. We demonstrate a simple microfluidic technology, SurfaceChIP-seq, for profiling genome-wide histone modifications using as few as 30 to 100 cells per assay and with up to eight assays running in parallel. We applied the technology to profile epigenomes using nuclei isolated from prefrontal cortex and cerebellum of mouse brain. Our cell type-specific data revealed that neuronal and glial fractions exhibited profound epigenomic differences across the two functionally distinct brain regions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.