Evidence map›Paper›PMID 29675316›Full record

ArticleBiomedical optics express2018

Widefield standing wave microscopy of red blood cell membrane morphology with high temporal resolution.

Peter W Tinning, Ross Scrimgeour, Gail McConnell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Peter W TinningDepartment of Physics, SUPA, University of Strathclyde, Glasgow, G4 ONG, UK.
Ross ScrimgeourDepartment of Physics, SUPA, University of Strathclyde, Glasgow, G4 ONG, UK.
Gail McConnellDepartment of Physics, SUPA, University of Strathclyde, Glasgow, G4 ONG, UK.

Funding

Medical Research Council MR/K015583/1
6 · The paper itself

Abstract

We report the first demonstration of widefield standing wave (SW) microscopy of fluorescently labelled red blood cells at high speeds that allow for the rapid imaging of membrane deformations. Using existing and custom MATLAB functions, we also present a method to generate 2D and 3D reconstructions of the SW data for improved visualization of the cell. We compare our technique with standard widefield epifluorescence imaging and show that the SW technique not only reveals more topographical information about the specimen but does so without increasing toxicity or the rate of photobleaching and could make this a powerful technique for the diagnosis or study of red blood cell morphology and biomechanical characteristics.

Indexed as

(100.3020) Image reconstruction-restoration(170.0180) Microscopy(170.2520) Fluorescence microscopy(170.3880) Medical and biological imaging(230.3670) Light-emitting diodes

Identifiers

PMID29675316
PMCPMC5905920

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.