ArticleFrontiers in plant science2018
Homology-Directed Repair of a Defective
Article in Frontiers in plant science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Binary vector copy number engineering improves Agrobacterium-mediated transformation.Nature biotechnology · 2025Article
- Beyond a few bases: methods for large DNA insertion and gene targeting in plants.The Plant journal : for cell and molecular biology · 2025Review
- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
- From Petri Dish to Field: Plant Tissue Culture and Genetic Engineering of Oats for Improved Agricultural Outcomes.Plants (Basel, Switzerland) · 2023Review
- Mechanodetection of neighbor plants elicits adaptive leaf movements through calcium dynamics.Nature communications · 2023Article
- CRISPR/Cas9-mediated homology donor repair base editing confers glyphosate resistance to rice (Frontiers in plant science · 2023Article
- Multi-allelic gene editing in an apomictic, tetraploid turf and forage grass (Frontiers in plant science · 2023Article
- Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA.Plant physiology · 2022Article
- An update on precision genome editing by homology-directed repair in plants.Plant physiology · 2022Article
- Gene targeting in polymerase theta-deficient Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2022Article
- Enhancing HR Frequency for Precise Genome Editing in Plants.Frontiers in plant science · 2022Article
- Article
- Gene Editing Technologies for Sugarcane Improvement: Opportunities and Limitations.Sugar tech : an international journal of sugar crops & related industries · 2022Article
- Genome Editing and Designer Crops for the Future.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Gene Targeting Facilitated by Engineered Sequence-Specific Nucleases: Potential Applications for Crop Improvement.Plant & cell physiology · 2021Review
- Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering.Transgenic research · 2021Review
- Sequence modification on demand: search and replace tools for precise gene editing in plants.Transgenic research · 2021Review
- Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a.Plant biotechnology journal · 2021Article
- Can genetic engineering-based methods for gene function identification be eclipsed by genome editing in plants? A comparison of methodologies.Molecular genetics and genomics : MGG · 2021Review
- Efficient base editing in tomato using a highly expressed transient system.Plant cell reports · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 system has emerged as a powerful tool for targeted genome editing in plants and beyond. Double-strand breaks induced by the Cas9 enzyme are repaired by the cell's own repair machinery either by the non-homologous end joining pathway or by homologous recombination (HR). While the first repair mechanism results in random mutations at the double-strand break site, HR uses the genetic information from a highly homologous repair template as blueprint for repair of the break. By offering an artificial repair template, this pathway can be exploited to introduce specific changes at a site of choice in the genome. However, frequencies of double-strand break repair by HR are very low. In this study, we compared two methods that have been reported to enhance frequencies of HR in plants. The first method boosts the repair template availability through the formation of viral replicons, the second method makes use of an
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