ArticleNature protocols2018
Preparation of a Nile Red-Pd-based fluorescent CO probe and its imaging applications in vitro and in vivo.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Unlocking the dual roles of carbon monoxide by a rapid fluorescent probe: from monitoring pneumonia therapy in animals to cadmium resistance in plants.Mikrochimica acta · 2026Article
- Bridging bench to bedside: a dual-use framework for chromene-based anti-obesity and antiviral therapeutics.Frontiers in pharmacology · 2025Article
- BODIPY Based OFF-ON Fluorescent Probe for Endogenous Carbon Monoxide Imaging in Living Cells.Journal of fluorescence · 2024Article
- Luminescence and Palladium: The Odd Couple.Molecules (Basel, Switzerland) · 2023Review
- Luminescent Metal Complexes as Emerging Tools for Lipid Imaging.Topics in current chemistry (Cham) · 2022Review
- Activity-Based Sensing: A Synthetic Methods Approach for Selective Molecular Imaging and Beyond.Angewandte Chemie (International ed. in English) · 2020Review
- CO Sense and Release Flavonols: Progress toward the Development of an Analyte Replacement PhotoCORM for Use in Living Cells.ACS omega · 2020Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbon monoxide (CO) is a key gaseous signaling molecule in living cells and organisms. This protocol illustrates the synthesis of a highly sensitive Nile Red (NR)-Pd-based fluorescent probe, NR-PdA, and its applications for detecting endogenous CO in tissue culture cells, ex vivo organs, and zebrafish embryos. In the NR-PdA synthesis process, 3-diethylamine phenol reacts with sodium nitrite in the acidic condition to afford 5-(diethylamino)-2-nitrosophenol hydrochloride (compound 1), which is further treated with 1-naphthalenol at a high temperature to provide the NR dye via a cyclization reaction. Finally, NR is reacted with palladium acetate to obtain the desired Pd-based fluorescent probe NR-PdA. NR-PdA possesses excellent two-photon excitation and near-IR emission properties, high stability, low background fluorescence, and a low detection limit. In addition to the chemical synthesis procedures, we provide step-by-step procedures for imaging endogenous CO in RAW 264.7 cells, mouse organs ex vivo, and live zebrafish embryos. The synthesis process for the probe requires ∼4 d, and the biological imaging experiments take ∼14 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.