Evidence map›Paper›PMID 29673588›Full record

ArticleBiochemical and biophysical research communications2018

Cdc42-dependent modulation of rigidity sensing and cell spreading in tumor repopulating cells.

Farhan Chowdhury, Sultan Doğanay, Benjamin J Leslie, Rishi Singh, Kshitij Amar, Bhavana Talluri, Seongjin Park, Ning Wang, Taekjip Ha

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. DeepScratch: Single-cell based topological metrics of scratch wound assays.Computational and structural biotechnology journal · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Farhan ChowdhuryDepartment of Mechanical Engineering and Energy Processes, Southern Illinois University Carbondale, Carbondale, IL 62901, USA. Electronic address: farhan.chowdhury@siu.edu.
Sultan DoğanayDepartment of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Benjamin J LeslieHoward Hughes Medical Institute, Johns Hopkins University, Baltimore, MD 21205, USA.
Rishi SinghDepartment of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Kshitij AmarDepartment of Mechanical Engineering and Energy Processes, Southern Illinois University Carbondale, Carbondale, IL 62901, USA.
Bhavana TalluriDepartment of Mechanical Engineering and Energy Processes, Southern Illinois University Carbondale, Carbondale, IL 62901, USA.
Seongjin ParkDepartment of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Ning WangDepartment of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Taekjip HaHoward Hughes Medical Institute, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA.

Funding

Bioengineering to map stress propagation in cytoskeletonR01GM072744 · NIGMS · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI Ning Wang · 2005 to 2026
$7.6M
Howard Hughes Medical InstituteNIGMS NIH HHS R01 GM072744
6 · The paper itself

Abstract

Recently, a robust mechanical method has been established to isolate a small subpopulation of highly tumorigenic tumor repopulating cells (TRCs) from parental melanoma cells. In order to characterize the molecular and mechanical properties of TRCs, we utilized the tension gauge tether (TGT) single-molecule platform and investigated force requirements during early cell spreading events. TRCs required the peak single molecular tension of around 40 pN through integrins for initial adhesion like the parental control cells, but unlike the control cells, they did not spread and formed very few mature focal adhesions (FAs). Single molecule resolution RNA quantification of three Rho GTPases showed that downregulation of Cdc42, but not Rac1, is responsible for the unusual biophysical features of TRCs and that a threshold level of Cdc42 transcripts per unit cell area is required to initiate cell spreading. Cdc42 overexpression rescued TRC spreading through FA formation and restored the sensitivity to tension cues such that TRCs, like parental control cells, increase cell spreading with increasing single-molecular tension cues. Our single molecule studies identified an unusual biophysical feature of suppressed spreading of TRCs that may enable us to distinguish TRC population from a pool of heterogeneous tumor cell population.

Indexed as

Cell MovementAnimalsBiomechanical Phenomenacdc42 GTP-Binding ProteinFocal AdhesionsMelanoma, ExperimentalMicerho GTP-Binding ProteinsRNA, MessengerSingle Molecule Imagingcdc42 GTP-Binding Proteinrho GTP-Binding ProteinsRNA, MessengerCell adhesion and spreadingFocal adhesionssmFISHTension gauge tethersTumor repopulating cells

Identifiers

PMID29673588
PMCPMC6133653

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.