ArticleMethods in enzymology2018
Genetic Reporters of Neuronal Activity: c-Fos and G-CaMP6.
Article in Methods in enzymology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- SEX-DEPENDENT MODULATION OF WHOLE BRAIN cFOS EXPRESSION BY LIGHT AND MELANOPSIN IN THE MOUSE.bioRxiv : the preprint server for biology · 2026Article
- Mice lacking β-arrestin-2 in melanocortin 4 receptor-expressing neurons show marked metabolic deficits.JCI insight · 2026Article
- Article
- Therapeutic reactivation of hippocampal progenitors reverse cognition decline in mice with progressive brain demyelination.Stem cell reports · 2026Article
- Advancing glioblastoma research by establishing a whole brain slice model.Frontiers in oncology · 2026Article
- Remembering the opponent: neuronal activation associated with social memory in zebrafish.Frontiers in veterinary science · 2026Article
- Immunoreactivity and distribution of Fos in the mouse brain following diverse general anesthetics.Scientific reports · 2025Article
- Gpr37 modulates the severity of inflammation-induced GI dysmotility by regulating enteric reactive gliosis.iScience · 2025Article
- Human Umbilical Cord Mesenchymal Stem Cells Ameliorated Chronic Unpredictable Mild Stress-Induced Depression and Anxiety by Alleviating Neuroinflammation.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2025Article
- Mapping of c-Fos Expression in Rat Brain Sub/Regions Following Chronic Social Isolation: Effective Treatments of Olanzapine, Clozapine or Fluoxetine.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Role of Membrane Estrogen Receptor Alpha on the Positive Feedback of Estrogens on Kisspeptin and GnRH Neurons.eNeuro · 2024Article
- Article
- Changes in ethanol effects in knock-in mice expressing ethanol insensitive alpha1 and alpha2 glycine receptor subunits.Life sciences · 2024Article
- Noradrenergic tone is not required for neuronal activity-induced rebound sleep in zebrafish.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Article
- Genetic Ablation of Dentate Hilar Somatostatin-Positive GABAergic Interneurons is Sufficient to Induce Cognitive Impairment.Molecular neurobiology · 2024Article
- Using deep learning to quantify neuronal activation from single-cell and spatial transcriptomic data.Nature communications · 2024Article
- Delayed estrogen actions diminish food consumption without changing food approach, motor activity, or hypothalamic activation elicited by corticostriatal µ-opioid signaling.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Dopamine-mediated striatal activity and function is enhanced in GlyRα2 knockout animals.iScience · 2023Article
- Anterior hypothalamic parvalbumin neurons are glutamatergic and promote escape behavior.Current biology : CB · 2023Article
- A test of the social behavior network reveals differential patterns of neural responses to social novelty in bonded, but not non-bonded, male prairie voles.Hormones and behavior · 2023Article
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1 author.
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Abstract
The majority of 20th century investigations into anesthetic effects on the nervous system have used electrophysiology. Yet some fundamental limitations to electrophysiologic recordings, including the invasiveness of the technique, the need to place (potentially several) electrodes in every site of interest, and the difficulty of selectively recording from individual cell types, have driven the development of alternative methods for detecting neuronal activation. Two such alternative methods with cellular scale resolution have matured in the last few decades and will be reviewed here: the transcription of immediate early genes, foremost c-fos, and the influx of calcium into neurons as reported by genetically encoded calcium indicators, foremost GCaMP6. Reporters of c-fos allow detection of transcriptional activation even in deep or distant nuclei, without requiring the accurate targeting of multiple electrodes at long distances. The temporal resolution of c-fos is limited due to its dependence upon the detection of transcriptional activation through immunohistochemical assays, though the development of RT-PCR probes has shifted the temporal resolution of the assay when tissues of interest can be isolated. GCaMP6 has several isoforms that trade-off temporal resolution for signal to noise, but the fastest are capable of resolving individual action potential events, provided the microscope used scans quickly enough. GCaMP6 expression can be selectively targeted to neuronal populations of interest, and potentially thousands of neurons can be captured within a single frame, allowing the neuron-by-neuron reporting of circuit dynamics on a scale that is difficult to capture with electrophysiology, as long as the populations are optically accessible.
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