ArticleEnvironmental science & technology2018
Agglomeration of Escherichia coli with Positively Charged Nanoparticles Can Lead to Artifacts in a Standard Caenorhabditis elegans Toxicity Assay.
Article in Environmental science & technology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Article
- Comparative analysis of nanosilver toxicity in C. elegans: influence of exposure media on accumulation, physiological and biochemical effects.Environmental science and pollution research international · 2026Article
- Importance of control experiments in toxicity test of metal-organic frameworks: Recent advances and challenges.Eco-Environment & Health · 2025Review
- Article
- Nanoplastics-mediated physiologic and genomic responses in pathogenic Escherichia coli O157:H7.Journal of nanobiotechnology · 2025Article
- Potential Artifacts and Control Experiments in Toxicity Tests of Nanoplastic and Microplastic Particles.Environmental science & technology · 2022Review
- Current ecotoxicity testing needs among selected U.S. federal agencies.Regulatory toxicology and pharmacology : RTP · 2022Review
- Neurotoxicity Evaluation of Nanomaterials Using C. elegans: Survival, Locomotion Behaviors, and Oxidative Stress.Current protocols · 2022Article
- Article
- Acute exposure to gold nanoparticles aggravates lipopolysaccharide-induced liver injury by amplifying apoptosis via ROS-mediated macrophage-hepatocyte crosstalk.Journal of nanobiotechnology · 2022Article
- U.S. Federal Agency interests and key considerations for new approach methodologies for nanomaterials.ALTEX · 2022Article
- Multifunctional Gold Nanoparticles in Cancer Diagnosis and Treatment.International journal of nanomedicine · 2022Review
- Functionalized Antimicrobial Nanofibers: Design Criteria and Recent Advances.Journal of functional biomaterials · 2021Review
- In-house validation of AF4-MALS-UV for polystyrene nanoplastic analysis.Analytical and bioanalytical chemistry · 2021Article
- Micro and Nanoplastics Identification: Classic Methods and Innovative Detection Techniques.Frontiers in toxicology · 2021Review
- Parametric Optimization of an Air-Liquid Interface System for Flow-Through Inhalation Exposure to Nanoparticles: Assessing Dosimetry and Intracellular Uptake of CeONanomaterials (Basel, Switzerland) · 2020Article
- Selection of an Optimal Abrasion Wheel Type for Nano-Coating Wear Studies under Wet or Dry Abrasion Conditions.Nanomaterials (Basel, Switzerland) · 2020Article
- Article
- Strategies for robust and accurate experimental approaches to quantify nanomaterial bioaccumulation across a broad range of organisms.Environmental science. Nano · 2019Article
- Gold Nanoparticles in Diagnostics and Therapeutics for Human Cancer.International journal of molecular sciences · 2018Review
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
The increased use and incorporation of engineered nanoparticles (ENPs) in consumer products requires a robust assessment of their potential environmental implications. However, a lack of standardized methods for nanotoxicity testing has yielded results that are sometimes contradictory. Standard ecotoxicity assays may work appropriately for some ENPs with minimal modification but produce artifactual results for others. Therefore, understanding the robustness of assays for a range of ENPs is critical. In this study, we evaluated the performance of a standard Caenorhabditis elegans ( C. elegans) toxicity assay containing an Escherichia coli ( E. coli) food supply with silicon, polystyrene, and gold ENPs with different charged coatings and sizes. Of all the ENPs tested, only those with a positively charged coating caused growth inhibition. However, the positively charged ENPs were observed to heteroagglomerate with E. coli cells, suggesting that the ENPs impacted the ability of nematodes to feed, leading to a false positive toxic effect on C. elegans growth and reproduction. When the ENPs were tested in two alternate C. elegans assays that did not contain E. coli, we found greatly reduced toxicity of ENPs. This study illustrates a key unexpected artifact that may occur during nanotoxicity assays.
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Registered trials
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