ArticleBiochemistry2018
Optochemical Control of Protein Localization and Activity within Cell-like Compartments.
Article in Biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Chemogenetic and optogenetic strategies for spatiotemporal control of split-enzyme-based calcium recording.bioRxiv : the preprint server for biology · 2025Article
- Interfacing bacterial microcompartment shell proteins with genetically encoded condensates.Protein science : a publication of the Protein Society · 2025Article
- Synthetic control of actin polymerization and symmetry breaking in active protocells.Science advances · 2024Article
- Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences.Biochemistry · 2022Article
- Amphiphilic proteins coassemble into multiphasic condensates and act as biomolecular surfactants.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Optical Control of Phosphoinositide Binding: Rapid Activation of Subcellular Protein Translocation and Cell Signaling.ACS synthetic biology · 2021Article
- Designer membraneless organelles sequester native factors for control of cell behavior.Nature chemical biology · 2021Article
- Patterning Microtubule Network Organization Reshapes Cell-Like Compartments.ACS synthetic biology · 2021Article
- HaloFlippers: A General Tool for the Fluorescence Imaging of Precisely Localized Membrane Tension Changes in Living Cells.ACS central science · 2020Article
- Optically inducible membrane recruitment and signaling systems.Current opinion in structural biology · 2019Review
- Chemical modification of proteins with photocleavable groups.Methods in enzymology · 2019Article
- Synthetic cell-like membrane interfaces for probing dynamic protein-lipid interactions.Methods in enzymology · 2019Article
- Reversible Control of Protein Localization in Living Cells Using a Photocaged-Photocleavable Chemical Dimerizer.Journal of the American Chemical Society · 2018Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
We report inducible dimerization strategies for controlling protein positioning, enzymatic activity, and organelle assembly inside synthetic cell-like compartments upon photostimulation. Using a photocaged TMP-Haloligand compound, we demonstrate small molecule and light-induced dimerization of DHFR and Haloenzyme to localize proteins to a compartment boundary and reconstitute tripartite sfGFP assembly. Using photocaged rapamycin and fragments of split TEV protease fused to FRB and FKBP, we establish optical triggering of protease activity inside cell-size compartments. We apply light-inducible protease activation to initiate assembly of membraneless organelles, demonstrating the applicability of these tools for characterizing cell biological processes in vitro. This modular toolkit, which affords spatial and temporal control of protein function in a minimal cell-like system, represents a critical step toward the reconstitution of a tunable synthetic cell, built from the bottom up.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.