Evidence map›Paper›PMID 29671273›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscopy.

Nathan Y Bentolila, Raymond L Barnhill, Claire Lugassy, Laurent A Bentolila

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Textures of the tumour microenvironment.Essays in biochemistry · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Nathan Y BentolilaGenesis Innovation Lab, YULA School, Los Angeles, CA, USA.
Raymond L BarnhillDepartment of Pathology, Institut Curie, University of Paris René Descartes, Paris, France.
Claire LugassyDepartment of Translational Research, Institut Curie, Paris, France.
Laurent A BentolilaCalifornia NanoSystems Institute, University of California Los Angeles, Los Angeles, CA, USA. lbento@chem.ucla.edu.
California NanoSystems Institute · USInstitut Curie · FRUniversité Paris Cité · FR

Funding

UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
NCATS NIH HHS UL1 TR000124NCATS NIH HHS UL1 TR001881
6 · The paper itself

Abstract

Noninvasive imaging of reporter gene expression by two-photon excitation (2PE) laser scanning microscopy is uniquely suited to perform dynamic and multidimensional imaging down to single-cell detection sensitivity in vivo in deep tissues. Here we used 2PE microscopy to visualize green fluorescent protein (GFP) as a reporter gene in human melanoma cells implanted into the dermis of the mouse ear skin. We first provide a step-by-step methodology to set up a 2PE imaging model of the mouse ear's skin and then apply it for the observation of the primary tumor and its associated vasculature in vivo. This approach is minimally invasive and allows repeated imaging over time and continuous visual monitoring of malignant growth within intact animals. Imaging fluorescence reporter gene expression in small living animals by 2PE provides a unique tool to investigate critical pathways and molecular events in cancer biology such as tumorigenesis and metastasis in vivo with high-spatial and temporal resolutions.

Indexed as

AnimalsCell Culture TechniquesCell Line, TumorDermisEar, ExternalGenes, ReporterGreen Fluorescent ProteinsHumansInjections, IntradermalIntravital MicroscopyMelanomaMiceMice, NudeMicroscopy, ConfocalMicroscopy, Fluorescence, MultiphotonSkin NeoplasmsGreen Fluorescent ProteinsAngiotropismBlood vesselCancer cell migrationDermisIntravital imagingMelanomaMetastasisMouse earMultiphotonReporter gene expressionTwo-photon microscopy

Identifiers

PMID29671273
PMCPMC6658097
OpenAlexW2800999917

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.