Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii.

Crysten E Blaby-Haas, M Dudley Page, Sabeeha S Merchant

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.4field-weighted citation impact, top 43% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Crysten E Blaby-HaasDepartment of Biology, Brookhaven National Laboratory, Upton, NY, USA. cblaby@bnl.gov.

M Dudley PageDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.

Sabeeha S MerchantDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.

University of California, Los Angeles · USBrookhaven National Laboratory · US

Funding

Nutritional Copper Signaling and HomeostasisR37GM042143 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MERCHANT, SABEEHA · 2011 to 2020

$4.3M

NIGMS NIH HHS R37 GM042143

6 · The paper itself

Abstract

The unicellular green alga Chlamydomonas reinhardtii is a valuable experimental system in plant biology for studying metal homeostasis. Analyzing transcriptional regulation with promoter-fusion constructs in C. reinhardtii is a powerful method for connecting metal-responsive regulation with cis-regulatory elements, but overcoming expression-level variability between transformants and optimizing experimental conditions can be laborious. Here, we provide detailed protocols for the high-throughput cultivation of C. reinhardtii and assaying Venus fluorescence as a reporter for promoter activity. We also describe procedural considerations for relating metal supply to transcriptional activity.

Indexed as

Bacterial ProteinsBiological AssayChlamydomonas reinhardtiiGene Expression RegulationGene FusionGenes, ReporterGene Transfer TechniquesLuminescent ProteinsResponse ElementsBacterial ProteinsLuminescent Proteinsyellow fluorescent protein, BacteriaAlgaeCopperIronYellow fluorescent proteinZinc

Identifiers

PMID29671268

DOI10.1007/978-1-4939-7724-6_10

PMCPMC6448394

OpenAlexW2801700244

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.