Evidence map›Paper›PMID 29671154›Full record

ArticleAnnals of biomedical engineering2018

Cell Mimicking Microparticles Influence the Organization, Growth, and Mechanophenotype of Stem Cell Spheroids.

Nicholas R Labriola, Jessica S Sadick, Jeffrey R Morgan, Edith Mathiowitz, Eric M Darling

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of biomedical engineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 2 pooled it
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Nicholas R LabriolaCenter for Biomedical Engineering, Brown University, 175 Meeting Street, Box G-B397, Providence, RI, 02912, USA.
Jessica S SadickDepartment of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI, USA.
Jeffrey R MorganCenter for Biomedical Engineering, Brown University, 175 Meeting Street, Box G-B397, Providence, RI, 02912, USA.
Edith MathiowitzCenter for Biomedical Engineering, Brown University, 175 Meeting Street, Box G-B397, Providence, RI, 02912, USA.
Eric M DarlingCenter for Biomedical Engineering, Brown University, 175 Meeting Street, Box G-B397, Providence, RI, 02912, USA. Eric_Darling@brown.edu.
Brown University · US

Funding

COBRE for Skeletal Health and RepairP20GM104937 · NIGMS · RHODE ISLAND HOSPITAL · PI CHEN, QIAN · 2012 to 2016
$10.8M
Pilot Projects ProgramP30GM122732 · NIGMS · RHODE ISLAND HOSPITAL · PI CHEN, QIAN · 2017 to 2021
$6.6M
High-yield, lineage-specific enrichment of living mesenchymal stem cellsR01AR063642 · NIAMS · BROWN UNIVERSITY · PI DARLING, ERIC M · 2013 to 2018
$1.8M
National Institute of Arthritis and Musculoskeletal and Skin Diseases R01 AR063642National Institute of General Medical Sciences P20 GM104937National Science Foundation CAREER Award CBET1253189NIAMS NIH HHS R01 AR063642NIGMS NIH HHS P20 GM104937NIGMS NIH HHS P30 GM122732
6 · The paper itself

Abstract

Substrate stiffness is known to alter cell behavior and drive stem cell differentiation, though most research in this area has been restricted to traditional, two-dimensional culture systems rather than more physiologically relevant, three-dimensional (3D) platforms. In this study, we utilized polymer-based, cell mimicking microparticles (CMMPs) to deliver distinct, stable mechanical cues to human adipose derived stem cells in 3D spheroid culture to examine changes in adipogenic differentiation response and mechanophenotype. After 21 days of adipogenic induction, spheroids containing CMMPs (composite spheroids) stiffened in accordance with CMMP elasticity such that spheroids containing the stiffest, ~ 10 kPa, CMMPs were over 27% stiffer than those incorporating the most compliant, ~ 0.25 kPa CMMPs. Adipogenically induced, cell-only spheroids were over 180% larger and 50% more compliant than matched controls. Interestingly, composite spheroids cultured without chemical induction factors dissociated when presented with CMMPs stiffer than ~ 1 kPa, while adipogenic induction factors mitigated this behavior. Gene expression for PPARG and FABP4 were upregulated more than 45-fold in adipogenically induced samples compared to controls but were unaffected by CMMP elasticity, attributed to insufficient cell-CMMP contacts throughout the composite spheroid. In summary, mechanically tuned CMMPs influenced whole-spheroid mechanophenotype and stability but minimally affected differentiation response.

Indexed as

Gene Expression RegulationMechanotransduction, CellularAdipose TissueFemaleHumansMiddle AgedSpheroids, CellularStem Cells3D spheroid cultureAdipogenesisAtomic force microscopy (AFM)Elastic and viscoelastic propertiesHyper-compliant microparticlesSelf-assemblyStem cell differentiationTissue engineering

Identifiers

PMID29671154
PMCPMC6039261
OpenAlexW2802203710

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.