ArticleRice (New York, N.Y.)2018
Identification of a cold-tolerant locus in rice (Oryza sativa L.) using bulked segregant analysis with a next-generation sequencing strategy.
Article in Rice (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genome-wide lncRNAs identification and association analysis for cold-responsive genes at the booting stage in rice (Oryza sativa L.).The plant genome · 2020Pooled it
- Genetic Interaction Between Bph50 and Bph51 Confers Resistance to Brown Planthopper in Rice.Rice (New York, N.Y.) · 2026Article
- FScientific reports · 2025Article
- Combined Bulked Segregant Analysis-Sequencing and Transcriptome Analysis to Identify Candidate Genes Associated with Cold Stress inInternational journal of molecular sciences · 2025Article
- Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3.Plant signaling & behavior · 2024Article
- Abiotic Stress in Rice: Visiting the Physiological Response and Its Tolerance Mechanisms.Plants (Basel, Switzerland) · 2023Review
- Mapping QTL associated with resistance toFrontiers in plant science · 2023Article
- Identification of salt stress-tolerant candidate genes in the BCFrontiers in plant science · 2023Article
- BSR and Full-Length Transcriptome Approaches Identified Candidate Genes for High Seed Ratio inCurrent issues in molecular biology · 2022Article
- QTL and Candidate Genes: Techniques and Advancement in Abiotic Stress Resistance Breeding of Major Cereals.International journal of molecular sciences · 2022Review
- Miniature Inverted-repeat Transposable Elements Drive Rapid MicroRNA Diversification in Angiosperms.Molecular biology and evolution · 2022Article
- PNGSeqR: An R Package for Rapid Candidate Gene Selection through Pooled Next-Generation Sequencing.Plants (Basel, Switzerland) · 2022Article
- OsWRKY115 on qCT7 links to cold tolerance in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- ITRAQ-based quantitative proteomic analysis ofBreeding science · 2022Article
- Identification of candidate genes and clarification of the maintenance of the green pericarp of weedy rice grains.Frontiers in plant science · 2022Article
- Harnessing the potential of bulk segregant analysis sequencing and its related approaches in crop breeding.Frontiers in genetics · 2022Review
- QTL analysis of tuber shape in a diploid potato population.Frontiers in plant science · 2022Article
- Genetic loci associated with winter survivorship in diverse lowland switchgrass populations.The plant genome · 2021Article
- Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis.Scientific reports · 2021Article
- Characterization and fine mapping of a new dwarf mutant in Brassica napus.BMC plant biology · 2021Article
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11 authors.
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Abstract
backgroundCold stress can cause serious abiotic damage that limits the growth, development and yield of rice. Cold tolerance during the booting stage of rice is a key factor that can guarantee a high and stable yield under cold stress. The cold tolerance of rice is controlled by quantitative trait loci (QTLs). Based on the complex genetic basis of cold tolerance in rice, additional efforts are needed to detect reliable QTLs and identify candidate genes. In this study, recombinant inbred lines (RILs) derived from a cross between a cold sensitive variety, Dongnong422, and strongly cold-tolerant variety, Kongyu131, were used to screen for cold-tolerant loci at the booting stage of rice.
resultsA novel major QTL, qPSST6, controlling the percent seed set under cold water treatment (PSST) under the field conditions of 17 °C cold water irrigation was located on the 28.4 cM interval on chromosome 6. Using the combination of bulked-segregant analysis (BSA) and next-generation sequencing (NGS) technology (Seq-BSA), a 1.81 Mb region that contains 269 predicted genes on chromosome 6 was identified as the candidate region of qPSST6. Two genes, LOC_Os06g39740 and LOC_Os06g39750, were annotated as "response to cold" by gene ontology (GO) analysis. qRT-PCR analysis revealed that LOC_Os06g39750 was strongly induced by cold stress. Haplotype analysis also demonstrate a key role of LOC_Os06g39750 in regulating the PSST of rice, suggesting that it was the candidate gene of qPSST6.
conclusionsThe information obtained in this study is useful for gene cloning of qPSST6 and for breeding cold-tolerant varieties of rice using marker assisted selection (MAS).
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