ArticleAMB Express2018
Ribosomal binding site sequences and promoters for expressing glutamate decarboxylase and producing γ-aminobutyrate in Corynebacterium glutamicum.
Article in AMB Express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Molecular adaptations and engineering of extremophiles for synthetic biology and biotechnological applications.Frontiers in microbiology · 2026Review
- Microbial chassis design and engineering for production of gamma-aminobutyric acid.World journal of microbiology & biotechnology · 2024Review
- A multi-plex protein expression system for production of complex enzyme formulations in Trichoderma reesei.Journal of industrial microbiology & biotechnology · 2023Article
- Precise and versatile microplate reader-based analyses of biosensor signals from arrayed microbial colonies.Frontiers in microbiology · 2023Article
- Translation initiation consistency betweenFrontiers in bioengineering and biotechnology · 2023Article
- Production of γ-Aminobutyrate (GABA) in RecombinantACS omega · 2022Article
- Metabolic engineering of Corynebacterium glutamicum for efficient production of optically pure (2R,3R)-2,3-butanediol.Microbial cell factories · 2022Article
- Cis-Element Engineering Promotes the Expression ofInternational journal of molecular sciences · 2022Article
- Article
- Importance of the 5' regulatory region to bacterial synthetic biology applications.Microbial biotechnology · 2021Review
- Construction and application of a CRISPR/Cas9-assisted genomic editing system for Corynebacterium glutamicum.AMB Express · 2021Article
- Recent progress in production of amino acid-derived chemicals using Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2021Review
- Development and comparison of cell-free protein synthesis systems derived from typical bacterial chassis.Bioresources and bioprocessing · 2021Article
- The Effect of Promoter and RBS Combination on the Growth and Glycogen Productivity of Sodium-Dependent Bicarbonate Transporter (SbtA) OverexpressingFrontiers in microbiology · 2021Article
- Schisandrin B exerts hypnotic effects in PCPA-treated rats by increasing hypothalamic 5-HT and γ-aminobutyric acid levels.Experimental and therapeutic medicine · 2020Article
- Construction of a 3A system from BioBrick parts for expression of recombinant hirudin variants III in Corynebacterium glutamicum.Applied microbiology and biotechnology · 2020Article
- Obtaining a series of native gradient promoter-5'-UTR sequences in Corynebacterium glutamicum ATCC 13032.Microbial cell factories · 2020Article
- Enhanced production of recombinant proteins in Corynebacterium glutamicum by constructing a bicistronic gene expression system.Microbial cell factories · 2020Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Glutamate decarboxylase (GAD) converts L-glutamate (Glu) into γ-aminobutyric acid (GABA). Corynebacterium glutamicum that expresses exogenous GAD gene, gadB2 or gadB1, can synthesize GABA from its own produced Glu. To enhance GABA production in C. glutamicum, ribosomal binding site (RBS) sequence and promoter were searched and optimized for increasing the expression efficiency of gadB2. R4 exhibited the highest strength among RBS sequences tested, with 6 nt the optimal aligned spacing (AS) between RBS and start codon. This combination of RBS sequence and AS contributed to gadB2 expression, increased GAD activity by 156% and GABA production by 82% compared to normal strong RBS and AS combination. Then, a series of native promoters were selected for transcribing gadB2 under optimal RBS and AS combination. P
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.