ArticleFrontiers in bioengineering and biotechnology2018
Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms.
Article in Frontiers in bioengineering and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Engineering ethanologenic bacteriumSynthetic and systems biotechnology · 2026Article
- Fungal endophytes and leaf litter fungi as sources of novel inhibitor-resistant cellulase for biofuel production: a basic study.3 Biotech · 2024Article
- Recent Advances in Food Waste Transformations into Essential Bioplastic Materials.Molecules (Basel, Switzerland) · 2024Review
- Transcriptomic analysis of formic acid stress response in Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2022Article
- A plasmid-freeFrontiers in bioengineering and biotechnology · 2022Article
- Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development.Biotechnology for biofuels · 2021Article
- Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate.Bioresources and bioprocessing · 2021Article
- The Magnesium Concentration in Yeast Extracts Is a Major Determinant Affecting Ethanol Fermentation Performance ofFrontiers in bioengineering and biotechnology · 2020Article
- Development and characterization of acidic-pH-tolerant mutants ofBiotechnology for biofuels · 2020Article
- Transcriptomic Profiles ofFrontiers in microbiology · 2020Article
- Development ofBiotechnology for biofuels · 2019Review
- Physiological mechanism of improved tolerance ofBiotechnology for biofuels · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this work was to identify inhibitors in pretreated lignocellulosic slurries, evaluate high-throughput screening strategies, and investigate the impact of inhibitors on potential hydrocarbon-producing microorganisms. Compounds present in slurries that could inhibit microbial growth were identified through a detailed analysis of saccharified slurries by applying a combination of approaches of high-performance liquid chromatography, GC-MS, LC-DAD-MS, and ICP-MS. Several high-throughput assays were then evaluated to generate toxicity profiles. Our results demonstrated that Bioscreen C was useful for analyzing bacterial toxicity but not for yeast. AlamarBlue reduction assay can be a useful high-throughput assay for both bacterial and yeast strains as long as medium components do not interfere with fluorescence measurements. In addition, this work identified two major inhibitors (furfural and ammonium acetate) for three potential hydrocarbon-producing bacterial species that include
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